The study aims to asses the ability of cell tests based on the analysis of the anti-Aspergillus cell responses and identify Aspergillus bronchitis with patients with cystic fibrosis. In addition, the study will evaluate the contribution of biological classification of aspergillosis according to criteria recently proposed by Baxter et al. compared to the classification used in clinical practice in the hospital of Montpellier.
Study Type
INTERVENTIONAL
Allocation
NA
Purpose
DIAGNOSTIC
Masking
NONE
Enrollment
68
Irmb/U1058
Montpellier, France
Number of CD4 T cell response against Aspergillus fumigatus in patients with cystis fibrosis
Analysis T cells response for identification of aspergillus bronchitis with cystic fibrosis patients
Time frame: 12 months
Cytokine level of TCD8
Qualitative Evaluation of cytokine level (IFNg, TNFa, IL10 and IL4) of TCD8 to identify ABPA and aspergillus bronchitis
Time frame: 12 months
Cytokine level CD4 cells
Qualitative Evaluation of cytokine level CD4 cells in order to identify ABPA and aspergillus bronchitis
Time frame: 12 months
Level of fumigatus specific IgE
Contribution of several biological tests improve classification of aspergillus bronchitis
Time frame: 12 months
Level of fumigatus specific IgG
Contribution of several biological tests improve classification of aspergillus bronchitis
Time frame: 12 months
Real-time PCR for aspergillus in sputum
Contribution of several biological tests improve classification of aspergillus bronchitis
Time frame: 12 months
Galactomannan by enzyme immune assay detection in sputum
Contribution of several biological tests improve classification of aspergillus bronchitis
Time frame: 12 months
Levels of cytokine (IFNg, IL10, IL4 and TNF) evaluated by flow cytometry
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Contribution of several biological tests improve classification of aspergillus bronchitis
Time frame: 12 months
Level of IL10 evaluated by Elispot dedicated to anti-aspergillus CD4 T cells
Contribution of several biological tests improve classification of aspergillus bronchitis
Time frame: 12 months
Flow cytometry techniques dedicated to anti-aspergillus CD4 T cell assessment
Time frame: 12 months
Level of IFNg evaluated by Elispot dedicated to anti-aspergillus CD4 T cells
Contribution of several biological tests improve classification of aspergillus bronchitis
Time frame: 12 months
Elispot dedicated to anti-aspergillus CD4 T cell
Time frame: 12 months