Aim of study is to determine reactive oxygen products and antioxidant capacity with rosacea patients; to measure metalloproteinase level and inflammatory markers of immune response and investigate their corelation with demodex infestation and also investigate the role of photoaging and sebum secretion in rosacea.
Rosacea is a common and chronic inflammatory disease characterized by facial erythema, papule, pustule and telangiectasia. Ultraviolet, Demodex mite, oxidative stress and antioxidant system disorders are emphasized in increased inflammation seen in rosacea. The aim of our study was to evaluate the status of oxidative stress, matrix metalloproteinase (MMP) and inflammatory markers in systemic circulation, their relationship with demodex density and investigate the role of photoaging and sebum secretion in rosacea. Forty patients with rosacea and age, gender and skin phenotype matched 40 healthy volunteers were enrolled in the study. Reflectance confocal microscopy (RCM) was used to calculate facial demodex density. The number of demodex, follicle, the number of mite per follicle, the number of infested follicle and the number of mite per infested follicle were calculated with RCM. Photoaging severity and facial sebum levels were also assessed. Total oxidant capacity (TOC), total antioxidant capacity (TAC), oxidative stress index (OSI), paraoxonase-1 (PON-1), aryl esterase (ARES), myeloperoxidase (MPO), tumor necrosis factor-alpha (TNF-α), interleukin-1beta (IL-1β), matrix metalloproteinase-1 (MMP-1) and matrix metalloproteinase-9 (MMP-9) levels in venous blood were measured.
Study Type
OBSERVATIONAL
Enrollment
80
Total oxidant capacity (TOC), total antioxidant capacity (TAC), oxidative stress index (OSI), paraoxonase-1 (PON-1), aryl esterase (ARES), myeloperoxidase (MPO), tumor necrosis factor-alpha (TNF-α), interleukin-1beta (IL-1β), matrix metalloproteinase-1 (MMP-1) and matrix metalloproteinase-9 (MMP-9) levels in venous blood were measured.
1mm2-sized 10 images were taken from right cheek and forehead; the number of demodex, follicle, the number of mite per follicle, the number of infested follicle and the number of mite per infested follicle were calculated with RCM. And photoaging severity were also assessed by using RCM.
Sebum rate at forehead and right cheek were evaluated with sebumeter
Istanbul Training and Research Hospital
Istanbul, Org. Abd. Nafiz Gürman, Turkey (Türkiye)
RECRUITINGSeverity of rosacea
Time frame: Rosacea patients who refer to criteria will be evaluated throughout 4 months.
The number of demodex of right cheek and forehead
Time frame: Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months.
The number of follicle of right cheek and forehead
Time frame: Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months.
The number of mite per follicle of right cheek and forehead
Time frame: Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months.
The number of infested follicle of right cheek and forehead
Time frame: Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months.
The number of mite per infested follicle of right cheek and forehead
Time frame: Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months.
Total oxidant capacity (TOC)
Time frame: Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months.
Total antioxidant capacity (TAC)
Time frame: Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months.
Oxidative stress index (OSI)
Time frame: Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months.
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Dermoscopic photoaging scale were assessed by using video dermoscopy.
Paraoxonase-1 (PON-1)
Time frame: Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months.
Aryl esterase (ARES)
Time frame: Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months.
Myeloperoxidase (MPO)
Time frame: Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months.
Tumor necrosis factor-alpha (TNF-α)
Time frame: Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months.
Interleukin-1beta (IL-1β)
Time frame: Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months.
Matrix metalloproteinase-1 (MMP-1)
Time frame: Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months.
Matrix metalloproteinase-1 (MMP-9)
Time frame: Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months.