Impact of COPD on Muscle Regeneration

Laval University27 enrolled

Overview

Muscle weakness and atrophy are important consequences of chronic obstructive pulmonary disease (COPD) and can affect both respiratory and peripheral muscles. Impaired muscle regeneration is thought to be implicated in the process of muscle atrophy. In this study the investigators will evaluate muscle regenerative potential of the Vastus Lateralis (VL) and diaphragm of patients with COPD, compared to patients with normal lung function.

Preliminary results suggest that the activation and differentiation processes of satellite cells isolated from the VL of patients with COPD could be altered. The investigators propose to compare the regenerative potential of the VL and the diaphragm in 15 patients with COPD and 15 individuals with normal lung function undergoing a thoracic surgery. From muscle biopsies, satellite cells will be isolated and cultured to assess their proliferation and differentiation rates, and the expression level of myogenic effectors (MyoD, Myf5, myogenin, and MRF4). Alterations in these measurements will confirm the hypotheses that the regenerative potential is lower in both muscle groups in patients with COPD compared to controls and that in COPD, the regenerative potential is lower in VL compared to the diaphragm.

Study Type

OBSERVATIONAL

Enrollment

Conditions

COPD

Interventions

Muscle biopsiesPROCEDURE

Muscle biopsies will allow acces to samples for observational studies. Vastus latetalis: needle biopsy (Bergstrom technique). Diaphragm: open biopsy performed by thoracic surgeon during lung surgery.

Eligibility

Sex: ALLMin age: 40 YearsMax age: 80 Years

Medical Language ↔ Plain English

Inclusion Criteria: * Patient with a scheduled thoracic surgery * ≥ 40 years * Smoking cessation ≥ 1 month * Smoking history ≥ 10 pack-year Exclusion Criteria: * Subjects who received either chemiotherapy or radiotherapy * Hypoxemia * Body weight loss ≥ 10% * Use of systemic corticosteroid in the last month

Outcomes

Primary Outcomes

Muscle satellite cell proliferation index

Isolation from muscle biopsies of satellite cell to evaluate their regeneration capacity by evaluating the proliferation capacity

Time frame: Sequential analyses performed over a period of 2 weeks after tissue collection.

Muscle satellite cell differentiation index

Isolation from muscle biopsies of satellite cell to evaluate their regeneration capacity by evaluating the differentiation capacity

Time frame: Sequential analyses performed over a period of 2 months after tissue collection.

Data from ClinicalTrials.gov

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