Trial of Adoptive T Cell Therapy With Activated P53 Specific T Cells for Treatment of Advanced Colorectal Cancer

Inclusion Criteria * Histologically confirmed UICC stage IV colorectal cancer and non resectable primary tumor and/or metastases * Previous palliative standard systemic treatment with FOLFOX, FOLFIRI, or FOLFIRINOX in combination with Bevacizumab or Cetuximab/Panitumumab, and currently under respective treatment * The following situation after at least four cycles with FOLFOX, FOLFIRI, or FOLFIRINOX in combination with Bevacizumab or Cetuximab/Panitumumab should apply: * Disease control (stable disease, partial response or complete response) according routine imaging * Resection of tumor and/or metastases still impossible * Tolerability of systemic treatment * The decision of the treating physician is to continue the standard background treatment * Presence of tumor-reactive T cells in the blood as detected by ex vivo IFN-γ EliSpot assay and classification of the reaction as a response in a blood sample * ECOG-performance status 0 or 1 * Adequate vein status at both cubital fossas for 16 G puncture (white permanent venous catheter, Braunüle; EN ISO 6009) * Age ≥ 18 years, any ethnic origin and gender * Ability of the patient to understand the character and individual consequences of the clinical trial * Patients should be willing and able to comply with scheduled visits, treatment plan, laboratory tests, and other study procedures * Written informed consent (must be available before enrolment in the trial) * Negative pregnancy test (females of childbearing potential) * Women with childbearing potential and male patients with partners of childbearing potential should be willing to use adequate contraception (failure rate less than 1% per year when used consistently and correctly) during the study and three month last application of T cell therapy * At the screening visit the following laboratory parameters apply and should be within the ranges specified: Lab Parameter Range WBC ≥4000/mm3 (≥4000/ul) Platelets ≥80.000/mm3 (≥80.000/ul) Creatinine ≤1.5mg/dl ALT, AST, and total bilirubin \< 2.5 x ULN HB\* \> 9 g/dl \*HB is controlled again at day -11 at DRK Mannheim as part of the procedures before leukapheris. Exclusion Criteria * Brain or leptomeningeal metastases, shown by MRI Scan during Screening phase or by routinely available other appropriate diagnostic (CT, MRI, PET) conducted within 3 months prior to inclusion. * Previous malignancy within the past 5 years. However: Patients who had curatively treated basal cell carcinoma of the skin, early gastrointestinal cancer treated by endoscopic resection and/or in situ carcinoma of the cervix are allowed for enrolment. * History of organ allograft or prior hematopoetic stem cell transplantation * History of inflammatory bowel disease (ulcerative colitis, Crohn's disease) * History of symptomatic autoimmune disease (e.g., rheumatoid arthritis, systemic progressive sclerosis \[scleroderma\], systemic lupus erythematosus, autoimmune vasculitis \[e.g., Wegener's Granulomatosis\]) * History of motor neuropathy considered of autoimmune origin (e.g., Guillain-Barré Syndrome) * Clinically significant cardiac disease (NYHA Class III or IV). * Other serious illnesses, which renders the patient unsuitable for study entry or multiple blood sampling * Serious intercurrent illness, requiring hospitalization * HIV and/or HBC/HCV positivity, tested by appropriate infection diagnostics during screening period * Any active infection or signs or symptoms of infection * Patient pregnant or breastfeeding * History of hypersensitivity to the investigational medicinal product or to any excipient present in the investigational medicinal product or to anti-coagulance auxiliary medicianal product (Heparin prophylaxis) * Treatment with immunosuppressive drugs (e.g. systemic corticosteroids) besides intermittent, anti-emetic steroid application during treatment with FOLFOX, FOLFIRI, or FOLFIRINOX in combination with Bevacizumab or Cetuximab/Panitumumab. Topical or inhalation steroids are permitted. * Treatment with any approved anti-cancer therapy within 28 days prior to enrolment, except the background treatment FOLFOX, FOLFIRI, or FOLFIRINOX in combination with Bevacizumab or Cetuximab/Panitumumab.as recommended by that trial protocol * Treatment with any live attenuated vaccine within 4 weeks before first administration of investigational medicinal product * Treatment with any other investigational agent or participation in another interventional clinical trial within 28 days prior to enrolment. No patient will be allowed to enroll in this trial more than once.