This research trial studies metabolic changes in blood samples from patients with acute myeloid leukemia. Studying samples of blood from patients with acute myeloid leukemia in the laboratory may help doctors learn more about cancer and the development of drug resistance.
PRIMARY OBJECTIVES: I. To determine the metabolic adaptations that occur following exposure to standard chemotherapeutic agents using peripheral blood mononuclear cells from acute myeloid leukemia (AML) patients. OUTLINE: Patients undergo collection of peripheral blood mononuclear cell (PBMC) samples for analysis via cell isolation, glycolytic flux and oxygen consumption assays, viability assays, and western blot at baseline, 24, 48, and 72 hours after starting chemotherapy.
Study Type
OBSERVATIONAL
Enrollment
14
Undergo collection of PBMC samples
Undergo metabolic analysis
Comprehensive Cancer Center of Wake Forest University
Winston-Salem, North Carolina, United States
Glycolytic flux rate
Results will be analyzed using descriptive statistics. Comparison between groups will be done using t-tests or ANOVA procedures. Other inferential statistical analysis will be conducted as appropriate.
Time frame: Up to 72 hours after starting chemotherapy
Level of fatty acid synthase by western blot
Results will be analyzed using descriptive statistics. Comparison between groups will be done using t-tests or ANOVA procedures. Other inferential statistical analysis will be conducted as appropriate.
Time frame: Up to 72 hours after starting chemotherapy
Number of mononuclear cells per sample determined by cell isolation
Results will be analyzed using descriptive statistics. Comparison between groups will be done using t-tests or analysis of variance (ANOVA) procedures. Other inferential statistical analysis will be conducted as appropriate.
Time frame: Up to 72 hours after starting chemotherapy
Oxygen consumption rate
Results will be analyzed using descriptive statistics. Comparison between groups will be done using t-tests or ANOVA procedures. Other inferential statistical analysis will be conducted as appropriate.
Time frame: Up to 72 hours after starting chemotherapy
Number of viable samples
Results will be analyzed using descriptive statistics. Comparison between groups will be done using t-tests or ANOVA procedures. Other inferential statistical analysis will be conducted as appropriate.
Time frame: Up to 72 hours after starting chemotherapy
Level of hexokinase by western blot
Results will be analyzed using descriptive statistics. Comparison between groups will be done using t-tests or ANOVA procedures. Other inferential statistical analysis will be conducted as appropriate.
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Time frame: Up to 72 hours after starting chemotherapy
Level of phosphofructokinase-1 by western blot
Results will be analyzed using descriptive statistics. Comparison between groups will be done using t-tests or ANOVA procedures. Other inferential statistical analysis will be conducted as appropriate.
Time frame: Up to 72 hours after starting chemotherapy
Level of phosphoglycerate mutase by western blot
Results will be analyzed using descriptive statistics. Comparison between groups will be done using t-tests or ANOVA procedures. Other inferential statistical analysis will be conducted as appropriate.
Time frame: Up to 72 hours after starting chemotherapy
Total level of pyruvate kinase M2 by western blot
Results will be analyzed using descriptive statistics. Comparison between groups will be done using t-tests or ANOVA procedures. Other inferential statistical analysis will be conducted as appropriate.
Time frame: Up to 72 hours after starting chemotherapy
Level of pyruvate kinase M2 for phospho-T105 by western blot
Results will be analyzed using descriptive statistics. Comparison between groups will be done using t-tests or ANOVA procedures. Other inferential statistical analysis will be conducted as appropriate.
Time frame: Up to 72 hours after starting chemotherapy
Level of phosphofructokinase-2 by western blot
Results will be analyzed using descriptive statistics. Comparison between groups will be done using t-tests or ANOVA procedures. Other inferential statistical analysis will be conducted as appropriate.
Time frame: Up to 72 hours after starting chemotherapy