Safety and Immunogenicity of HIV DNA-C CN54ENV and Recombinant HIV CN54gp140 Vaccines in Healthy Volunteers

Inclusion Criteria: 1. Men and women aged between 18 and 50 years on the day of screening 2. BMI between 18-30 3. Available for follow-up for the duration of the study (\~5 months from screening) 4. Willing and able to give written informed consent 5. At low risk of HIV and willing to remain so for the duration of the study defined as: * no history of injecting drug use in the previous ten years * no gonorrhoea or syphilis in the last six months * no high risk partner (e.g. injecting drug use, HIV positive partner) either currently or within the past six months * no unprotected anal intercourse in the last six months, outside a relationship with a regular partner known to be HIV negative * no unprotected vaginal intercourse in the last six months outside a relationship with a regular known/presumed HIV negative partner 6. Willing to undergo a HIV test 7. Willing to undergo a genital infection screen 8. Must agree to require male sexual partner to use condoms, from at least 14 days before the first vaccination until at least 14 days after the last 9. If heterosexually active female capable of becoming pregnant, must (in addition to requiring male partner to use condoms) agree to use hormonal contraception, or to complete abstinence, from at least 14 days before the first vaccination until at least 14 days after the last. \[Note: Periodic abstinence (e.g. calendar, ovulation, symptothermal, post-ovulation methods) and withdrawal, and IUD/IUS, are not acceptable methods of contraception.\] If sexually active male, must agree to use condoms from the day of first vaccination until at least 14 days after the last. \[Note: Additional use of an effective method of contraception is recommended for any non-pregnant female partner over the same period.\] 10. Agree to abstain from donating blood for three months after the end of their participation in the trial, or longer if necessary 11. Registered with a GP for at least the past three months 12. Entered and clearance obtained from The Over-volunteering Prevention System (TOPS) database. Exclusion Criteria: 1. Pregnant or lactating 2. History of cardiac arrhythmia or palpitations \[e.g., supraventricular tachycardia, atrial fibrillation, frequent ectopy, or sinus bradycardia prior to study entry (sinus arrhythmia is not excluded) 3. History of syncope or fainting episodes within 1 year of study entry 4. History of grand-mal epilepsy, seizure disorder or any history of prior seizure 5. Individuals in which a skin-fold measurement (cutaneous and subcutaneous tissue) of the upper right or left thigh exceeds 40 mm 6. Clinically relevant abnormality on history or examination 7. Known hypersensitivity to any component of the vaccine formulations used in this trial, or have severe or multiple allergies to drugs or pharmaceutical agents 8. History of severe local or general reaction to vaccination defined as * local: extensive, indurated redness and swelling involving most of the antero-lateral thigh or the major circumference of the arm, not resolving within 72 hours * general: fever ≥39.5 °C within 48 hours; anaphylaxis; bronchospasm; laryngeal oedema; collapse; convulsions or encephalopathy within 72 hours 9. Receipt of live attenuated vaccine or HIV envelope components within 60 days or other vaccines within 14 days of enrolment 10. Receipt of an experimental vaccine containing HIV envelope components at any time in the past 11. Receipt of blood products or immunoglobulin within 4 months of screening 12. Participation in another trial of a medicinal product, completed less than 30 days prior to enrolment. 13. HIV 1 or 2 positive or indeterminate on screening. 14. Positive for hepatitis B surface antigen, hepatitis C antibody or serology indicating active syphilis requiring treatment 15. Grade 1 or above routine laboratory parameters. Hyperbilirubinaemia to be considered an exclusion criterion only when confirmed to be conjugated bilirubinaemia 16. Current use of any electronic stimulation device, such as cardiac demand pacemakers, automatic implantable cardiac defibrillator, nerve stimulators, or deep brain stimulators. 17. Presence of any surgical or traumatic metal implants at the sites of administration 18. Unable to read and speak English to a fluency level adequate for the full comprehension of procedures required in participation and consent. 19. Women with a history of toxic shock syndrome. 20. Women using an intrauterine device for contraception (as incompatible with softcup sampling) 21. Unlikely to comply with protocol.