Effect of Exosomes Derived From Red Blood Cell Units on Platelet Function and Blood Coagulation

Johann Wolfgang Goethe University Hospital18 enrolled

Overview

This study is designed to analyze the effect exosomes derived from red blood cell units have on blood coagulation and platelet function. It is an in vitro study using healthy volunteers' blood.

The blood of 25 healthy volunteers will be exposed to exosomes derived from red blood cell units. The effects on coagulation and platelet function will be measured using thromboelastometry (ROTEM®) and FACS analysis.

Study Type

INTERVENTIONAL

Allocation

Purpose

BASIC_SCIENCE

Masking

NONE

Enrollment

Conditions

Blood Coagulation Platelet Function

Interventions

in vitro studyOTHER

Exosomes will be mixed with healthy volunteers' blood in vitro

Eligibility

Sex: ALLMin age: 18 YearsMax age: 80 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * healthy volunteers Exclusion Criteria: * pregnancy * drug use

Locations (1)

University Hospital Frankfurt

Frankfurt am Main, Hesse, Germany

Outcomes

Primary Outcomes

clotting time

measured by thromboelastometry (ROTEM® Analysis)

Time frame: 2 months

Secondary Outcomes

clot formation

measured by thromboelastometry (ROTEM® Analysis)

Time frame: 1h after exposure

clot stability

measured by thromboelastometry (ROTEM® Analysis)

Time frame: 1h after exposure

clot lysis

measured by thromboelastometry (ROTEM® Analysis)

Time frame: 1h after exposure

CD62P

FACS analysis

Time frame: 3h after exposure

Data from ClinicalTrials.gov

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