Endocrine Regulation of Lipolysis in Obesity and Diabetes

Hasselt University55 enrolled

Overview

Adipose tissue (AT) dysfunction is a commonly observed feature of metabolic dysfunction in obesity and diabetes. An important characteristic when examining AT function is the capacity to break down stored lipids (i.e. lipolysis) and its regulation. In the present study, the aim was to investigate whether atrial natriuretic peptide-mediated lipolysis is altered in different adipose tissue depots (subcutaneous and visceral adipose tissue) of obese subjects with or without type 2 diabetes, compared to age-matched lean men. Eventually, the knowledge gained from this research will contribute to a better understanding of the present adipose tissue dysfunction and to the optimization of exercise programs for people with obesity and diabetes.

Study Type

INTERVENTIONAL

Allocation

NON_RANDOMIZED

Masking

NONE

Enrollment

Conditions

Obesity Diabetes

Interventions

Eligibility

Sex: MALEMin age: 40 YearsMax age: 65 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Lean group: BMI \< 25 kg/m², insulin sensitive (HOMA-Insuline Resistance (IR) \< 2.3), age 40-65 * Obese groups: BMI \> 30 kg/m², presence or absence of type 2 diabetes based on use of glucose lowering medication or HbA1c \> 6.5%, age 40-65 Exclusion Criteria: * presence of a history of heart, lung or kidney disease and/or presence of endocrine anomalies. Use of beta-blockade medication.

Locations (4)

Hasselt University

Diepenbeek, Belgium

Ziekenhuis Oost-Limburg

Genk, Belgium

Jessa Ziekenhuis

Hasselt, Belgium

Maastricht University

Maastricht, Netherlands

Outcomes

Primary Outcomes

Glycerol release by mature adipocytes, isolated from subcutaneous and visceral adipose tissue, upon stimulation with isoprenalin or ANP (ex vivo)

Investigation of concentration effect of ISO/ANP on lipolysis (glycerol release) by mature adipocytes isolated from adipose tissue biopsies, glycerol release is measured in the incubation medium following a 3 hour incubation period. Measured for adipose tissue (subcutaneous and visceral adipose tissue) of both lean and obese subjects

Time frame: day 1

ANP receptor expression profiles on mRNA and protein level in both adipose tissue depots for both lean and obese subjects.

Receptor expression determination in order to clarify the underlying mechanism responsible for possible differences in lipolytic responses.

Time frame: day 1

Adipose tissue inflammation

Adipose tissue inflammation (by means of FACS analysis) and its relation to insulin sensitivity and adipose tissue function in lean, obese and obese diabetics subjects.

Time frame: day 1

Secondary Outcomes

Body composition

Body composition (body fath percentage), measured by BIA

Time frame: day 1

BMI (kg/m²)

Time frame: day 1

body height (m)

Time frame: day 1

weight (kg)

Time frame: day 1

hip circumference (cm)

Time frame: Day 1

waist (cm)

Time frame: Day 1

blood glucose

Time frame: day 1

blood insuline

Time frame: day 1

blood FFA

Time frame: day 1

blood triglycerides

Time frame: day 1

blood glycated hemoglobin

Time frame: day 1

blood ANP

fasting blood sample to determine blood glucose, insulin, FFA, , glycated hemoglobin and ANP

Time frame: day 1

blood pressure (mmHg)

Time frame: Day 1

Concomitant medication

Time frame: Day 1