This study measures the plasma half-life of 25-hydroxyvitamin D3 (25(OH)D3) in non-pregnant-non-lactating women, pregnant women at 30 wk gestational age and at 3 months post-partum in women who are exclusively breast-feeding.
Study Type
INTERVENTIONAL
Allocation
NON_RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
NONE
Enrollment
49
At each measurement, women are given an oral dose of stable isotope labelled 25(OH)D3. Blood and urine samples are collected at baseline and on day 21 post-dose. Additional blood samples are collected post-dose on days 6,9,24,27 and 30.
Plasma concentration of stable isotope labeled 25(OH)D3
The plasma half-life of 25(OH)D3 is calculated from the slope (k) of the disappearance of the stable isotope labeled 25(OH)D3 against time using the equation half-life=0.693/k
Time frame: Samples collected on day 6, 9, 21, 24, 27 and 30 post-dose
Plasma concentration of vitamin D metabolites and related calciotropic hormones
Analytes: 25(OH)D3, 24,25(OH)2D3, 1,25(OH)2D, vitamin D binding protein, parathyroid hormone, albumin, ionised calcium, phosphate
Time frame: Fasting plasma samples collected at baseline and 21 days post-dose
Urinary concentration of calcium, phosphate, creatinine and cyclic adenosine monophosphate (cAMP)
2-hour fasting urine collection
Time frame: Samples collected at baseline and 21 days post-dose
Weighed dietary intake
Dietary nutrient intakes assessed by 2-day weighed food diary and nutrient intakes calculated using DINO (diet in nutrients out) analysis programme (Fitt et al, 2015)
Time frame: 2 days within 30 days of dose
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