This study evaluates the effect of cashew nut consumption on traditional and emerging markers of cardiovascular disease (CVD) risk and determines the usable energy content of a serving of cashew nuts for accurate food labelling.
The cardioprotective effects of nuts have been well documented in the scientific literature; however, most of the research has been done with almonds, walnuts, and pistachios, while studies with cashew nuts are lacking. In addition to the cardiovascular literature, our recent studies measuring the metabolizable energy of nuts have demonstrated that the measured energy value of almonds, pistachios, and walnuts is lower than that predicted using the Atwater factors. However, the measured energy value of cashew nuts is unknown. There are two distinct aims of this study - determining the effects of cashew nut consumption on risk factors of CVD, and determining the energy value of cashew nuts in the human diet. The cardiovascular effects of cashew nuts will be determined by measuring both traditional and emerging risk factors. The metabolizable energy value of cashew nuts will be calculated based on the chemical composition and energy content of the consumed diet and excreta. This will provide a better estimate of the energy value than simply calculating energy value based on Atwater factors.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
PREVENTION
Masking
DOUBLE
Enrollment
42
Participants will receive a controlled diet with 0 g/d of cashew nuts. Meals will be prepared using traditional American foods with a macronutrient composition representative of a typical American diet.
Participants will receive a controlled diet with 42 g/d of cashew nuts. Meals will be prepared using traditional American foods with a macronutrient composition representative of a typical American diet.
USDA Beltsville Human Nutrition Research Center
Beltsville, Maryland, United States
Change in lipid/lipoprotein profile will be measured in blood
The following will be measured in the blood on 2 consecutive days at the beginning and end of each 4-week diet period: lipids and lipoproteins, apolipoproteins, lipoprotein particle number/size, and proprotein convertase subtilisin/kexin type 9 (PCSK9).
Time frame: Week 0, end of diet period 1 (week 4), beginning of diet period 2 (week 6), end of diet period 2 (week 10)
Metabolizable energy will be measured based on energy content of the consumed diet and excreta
The measured metabolizable energy value of cashew nuts will be calculated based on the energy content of the consumed diet and excreta.
Time frame: Second week of diet period 1 (week 2)
Metabolizable energy will be measured based on energy content of the consumed diet and excreta
The measured metabolizable energy value of cashew nuts will be calculated based on the energy content of the consumed diet and excreta.
Time frame: Second week of diet period 2 (week 8)
Pulse Wave will be measured using Sphygmocor EXCEL
Pulse wave analysis and pulse wave velocity will be conducted.
Time frame: Week 0, end of diet period 1 (week 4), beginning of diet period 2 (week 6), end of diet period 2 (week 10)
Markers of vascular health will be measured in blood
Adhesion molecules and endothelin-1 will be measured in the blood.
Time frame: Week 0, end of diet period 1 (week 4), beginning of diet period 2 (week 6), end of diet period 2 (week 10)
Digestibility (%) will be calculated as (intake - excreted)/intake
Fat, protein, and fiber digestibility will be calculated (%) and the effect of cashew nuts on nutrient digestibility (%) will be assessed.
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Time frame: Second week of diet period 1 (week 2), second week of diet period 2 (week 8)
Change in systemic inflammation will be measured in blood
Markers of systemic inflammation will be measured, such as IL-6, TNF-alpha, serum amyloid A, and CRP.
Time frame: Week 0, end of diet period 1 (week 4), beginning of diet period 2 (week 6), end of diet period 2 (week 10)
Change in hemostasis will be measured in blood
Fibrinogen and factor VII will be measured in the blood.
Time frame: Week 0, end of diet period 1 (week 4), beginning of diet period 2 (week 6), end of diet period 2 (week 10)