Vigil™ + Nivolumab in Advanced Non-Small Cell Lung Cancer

Tissue Procurement Inclusion Criteria: Patients will be eligible for tissue procurement for the Vigil™ manufacturing process, if they meet all of the following criteria: 1. Histologically or cytologically confirmed diagnosis of NSCLC. 2. Age ≥ 18 years. 3. Locally advanced or metastatic disease that is progressive after one prior platinum-based systemic chemotherapy regimen 1. Adjuvant therapy will count as a line of therapy if administered within 6 months of relapse). 2. Subjects with EGFR or ALK mutations should also have received appropriate targeted therapy. 4. No systemic therapy, immunologic therapy or investigational therapy within 3 weeks and no radiation therapy within 1 week prior to tumor procurement for vaccine manufacture. 5. Planned standard of care surgical procedure (e.g., tumor biopsy or palliative resection or thoracentesis) and expected availability of a cumulative mass of \~10-30 grams tissue ("golf-ball" size) or pleural fluid estimated volume ≥ 500mL (must be primary tap) for immunotherapy manufacture. 6. At least one area of cancer, not intended for vaccine manufacture, that is measureable by RECIST 1.1 criteria. 7. At least one tumor, not intended for vaccine manufacture, that is considered appropriate for on-treatment biopsy. The same tumor may suffice for both on-treatment biopsy and RECIST 1.1 measurement so long as imaging occurs prior to biopsy. 8. ECOG Performance Status ≤ 1 9. Estimated survival ≥ 6 months. 10. Ability to understand and the willingness to sign a written informed consent document for tissue harvest. Tissue Procurement Exclusion Criteria: Patients meeting any of the following criteria are not eligible for tissue procurement for the Vigil™ manufacturing: 1. Any localized anticancer therapy (e.g., radiation, radiofrequency ablation, cryotherapy) to tumor intended for vaccine manufacture unless unequivocal evidence of post-treatment disease progression of the target tumor. 2. Medical condition requiring any form of chronic systemic immunosuppressive therapy (steroid or other) except physiologic replacement doses of hydrocortisone or equivalent (no more than 30 mg hydrocortisone or 10 mg prednisone equivalent daily for \< 30 days duration 3. Known history of other malignancy unless having undergone curative intent therapy without evidence of that disease for ≥ 3 years except cutaneous squamous cell and basal cell skin cancer, superficial bladder cancer, in situ cervical cancer or other in situ cancers are allowed if definitively resected. 4. Brain metastases unless treated with curative intent (gamma knife or surgical resection) and without evidence of progression for ≥ 4 months. 5. Any documented history of autoimmune disease with exception of Type 1 diabetes on stable insulin regimen, hypothyroidism on stable dose of replacement thyroid medication, vitiligo, or asthma not requiring systemic steroids. 6. Known history of allergies or sensitivities to gentamicin. 7. History of or current evidence of any condition (including medical, psychiatric or substance abuse disorder), therapy, or laboratory abnormality that might confound the results of the study, interfere with the patient's participation for the full duration of the study, or is not in the best interest of the patient to participate, in the opinion of the treating Investigator. 8. Known HIV or chronic Hepatitis B or C infection. 9. History of pneumonitis or interstitial lung disease. Study Enrollment Inclusion Criteria: Patients will be eligible for registration into the trial if they meet all of the following inclusion criteria: 1. Successful manufacturing of at least 4 vials of Vigil™. 2. ECOG Performance Status ≤ 1 3. Estimated survival ≥ 4 months. 4. Disease that is measurable by RECIST 1.1 criteria. 5. Adequate organ function as defined by the following laboratory values: Absolute granulocyte count ≥ 1,500/mm3 Absolute lymphocyte count ≥ 500/mm3 Platelets ≥ 75,000/mm3 Hemoglobin ≥ 9 g/dL Creatinine ≤ 1.5x institutional upper limit of normal Total bilirubin ≤ 1.5x institutional upper limit of normal AST(SGOT) and ALT(SGPT) ≤2x institutional upper limit of normal or ≤5x institutional upper limit of normal if liver metastases INR / PT and aPTT ≤ 1.5 x ULN (if not using anticoagulants) Immunological Thyroid Stimulating Hormone within institutional limits 6. Subject has recovered to CTCAE Grade 1 or better from all adverse events associated with prior therapy or surgery. Pre-existing motor or sensory neurologic pathology or symptoms must be recovered to CTCAE Grade 2 or better. 7. If female of childbearing potential, has a negative urine or serum pregnancy test. If the urine test is positive or cannot be confirmed as negative, a negative serum test will be required for study entry. 8. Investigator deems the patient to have a tumor appropriate for on-treatment biopsy and patient agrees to provide tissue biopsy at Cycle 5 (Week 9) for correlative studies. 9. Ability to understand and the willingness to sign a written informed protocol specific consent. Study Enrollment Exclusion Criteria: In addition to the procurement exclusion criteria, patients will NOT be eligible for study registration and enrollment if meeting any of the following criteria: 1. Any anti-neoplastic therapy between tissue procurement for vaccine manufacture and start of study therapy. 2. Live vaccine used for the prevention of infectious disease administered \< 30 days prior to the start of study therapy. 3. Post-surgery complication that in the opinion of the treating investigator would interfere with the patient's study participation or make it not in the best interest of the patient to participate.