Evaluation of Effectiveness and Mildness of Whole-natural Hand-wash Formulations

Inclusion Criteria: For effectiveness test: * Healthy subjects aged 18-60 (both sexes). * Subjects must have short natural nails. * Subjects must remove all jewelry from their hands and forearms before participating. * Subjects must not expose their hands to any antimicrobial products for 24 hours before the test (including antimicrobial containing dish soaps, shampoos, and body washes). * Subjects must don safety glasses and lab coat prior to participating. * Subjects will not be exposed to more than one challenge microorganisms per week and more than five challenge microorganisms in total. For mildness test: * Subject aged 18-60 * Subjects should be healthy adults from both sexes (preferably more women than men) * Subjects would be able to participate in one study per four weeks. Exclusion Criteria For effectiveness test: * Subjects cannot have any breaks or cuts in the skin of the hands or forearms. * Subjects cannot have a medical diagnosis of a physical condition. * Subjects cannot have allergies to detergents, hand hygiene products, perfume or any other substance they are going to be expose to. * Subjects must not be on antibiotics or have been on antibiotics in the past 14 days. * Subjects cannot participate if they have participated in other clinical trials within the past 14 days. * Subjects cannot have any other general health or skin issues not mentioned above. * Subjects cannot be pregnant or nursing. For mildness test: * pregnant or nursing subjects * Subjects with an allergy or a skin disease which interferes with the proper results of the tests. * Subjects who are taking medications which can interfere with the proper results of the tests. For example: systemic corticosteroids, topical steroids applied to the test area. * Subjects with sun or phototherapy induced tanning at the test area. * Subject who had been participating in allergy tests within four weeks prior to the present tests. * Subjects could not use cosmetics for the face area except for the use of lipstick and makeup around the eyes for two weeks before the test. * Subjects can use only mild soap and no more than once a day. * Subjects should avoid drinking alcohol, coffee, tea and smoking on the test day.