The role of oxidative stress in disease pathology is increasingly recognized. At present, the development of biomarkers of this state is in its infancy and the availability of clinically validated assays is lacking. This study will better determine normal values for specific biomarkers of oxidative stress. It will also investigate normal values of taurine, a natural occurring oxidative stress protectant. A primary and specific application of this data is for the evaluation of oxidative stress, levels of the natural protectant taurine, and of associated inflammation in the context of cystathionine β-synthase (ClinicalTrials.gov study: Oxidative Stress Markers in Inherited Homocystinuria and the Impact of Taurine).
This study is a one-time collection of blood and urine samples in healthy individuals. Subjects have to be fasting for 3 hours prior to the blood sampling. A maximum of 7.5 mLs of blood (1 and 1/5 teaspoon) and a urine sample will be collected.
Study Type
OBSERVATIONAL
Enrollment
22
Children's Hospital Colorado
Aurora, Colorado, United States
Oxidative stress marker: TBARS
Single Draw for one-time analysis
Time frame: Time 0
Metabolite antioxidant: Taurine
Single Draw for one-time analysis
Time frame: Time 0
Inflammation marker: Supper oxide dismutase (SOD)
Single Draw for one-time analysis
Time frame: Time 0
Inflammation marker: TGFβ
Single Draw for one-time analysis
Time frame: Time 0
Inflammation marker: myeloperoxidase
Single Draw for one-time analysis
Time frame: time 0
Metabolites: S-adenosylmethionine and S-adenosylhomocysteine
Single Draw for one-time analysis (same units)
Time frame: Time 0
Vascular function: Thromboxane B2 metabolites
Single Draw for one-time analysis (same units)
Time frame: Time 0
Oxidative stress: dityrosine
Single Draw for one-time analysis
Time frame: Time 0
Inflammation markers: IL1α, IL-1β, IL10, TNFα
Single Draw for one-time analysis; ELISA assay (same units)
Time frame: Time 0
Inflammation markers: cytokines: IL1α, IL-1β IL-1ra, IL6, IL8, IL17, MCP-1,MIP-1, MIP1α, MIPβ
Single Draw for one-time analysis; Luminex assay (same units)
Time frame: Time 0
Inflammation markers: cytokines: TNFα, IL4, IL10 and IL12
Single Draw for one-time analysis; high sensitivity Luminex assay (same units)
Time frame: Time 0
Inflammation marker: high sensitivity CRP
Single Draw for one-time analysis
Time frame: Time 0
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