To describe the effect and mechanisms of action faba bean powder and fractions (protein, starch and fiber) on glucose and insulin homeostasis, food intake, satiety and metabolic regulation in young adults (18 to 30 years).
The overall objective is to describe the effect and mechanisms of action of faba bean powder and fractions (protein, starch and fiber) on glucose and insulin homeostasis, food intake, satiety and metabolic regulation in young adults (18 to 30 years). The specific objectives are to test the acute effects of different faba bean ingredient smoothie on: 1) post-prandial blood glucose, appetite, satiety hormone and insulin for 200 minutes, and 2) food intake 120 minutes following consumption of the faba bean smoothie. Two short-term acute studies will be conducted to meet these objectives. Both acute studies will follow a randomized, double blinded, balanced, repeated measures design and the same protocol; except one will have an ad libitum pizza meal (study 1), while the other will have a fixed pizza meal (study 2) at 120 minutes. Half the participants will have blood drawn by IV and the other half will be finger prick.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
DOUBLE
Enrollment
60
Breakfast smoothie with 32 g whole faba bean powder (300g).
Breakfast smoothie with 32 g faba bean protein concentrate.
Breakfast smoothie with 33 g faba bean starch.
Richardson Centre for Functional Foods and Nutraceuticals
Winnipeg, Manitoba, Canada
blood glucose
measured by finger prick blood samples by glucose meters
Time frame: 0-200 minutes
appetite
measured by VAS questionnaire
Time frame: 0-200 minutes
satiety hormones
measured by enzyme-linked immunosorbent assay (ELISA)
Time frame: 0- 200mins
insulin
measured by ELISA
Time frame: 0-200mins
food intake
energy intake at ad-libitum pizza meal
Time frame: 120 minutes following consumption of the faba bean smoothie
physical comfort
measured by VAS questionnaire
Time frame: 0-200 minutes
energy/fatigue
measured by VAS questionaire
Time frame: 0-200 minutes
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Breakfast smoothie with 32 g faba bean protein isolate.
Control smoothie with 32 g corn maltodextrin without added faba bean fractions.