Air pollution is a global environmental and health concern, contributing to onset and deterioration of respiratory and cardiovascular diseases. As climate change and dependence on diminishing fossil fuel supplies have taken center stage in political and scientific debates, renewable carbon-neutral fuels like biodiesel receive increasing attention. The most common biodiesel within the European Union, rapeseed oil methyl ester (RME) is perceived to be a "green fuel", as it is sustainable and of biological origin, and therefore is often predicted to be less harmful to human health. Whilst replacing petrodiesel with biodiesel may have advantageous ecological impacts, consequences to respiratory health remained largely unexplored. The purpose of the current study is to evaluate whether inhalation of 100% RME biodiesel exhaust would result in an acute airway inflammatory response in healthy human subjects, as shown previously following exposure to petrodiesel exhaust.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
TRIPLE
Enrollment
15
The bronchoscopy procedure was performed six hours after completion of the exposure session, with sampling of endobronchial mucosal biopsies, bronchial wash and bronchoalveolar lavage performed under topical anaesthesia.
Cytokines in Bronchial wash (BW)
Bronchial wash (BW) collected by bronchoscopy will be analyzed for cytokines (%).
Time frame: 6 hours
Cell counts in Bronchial Wash
Bronchial wash (BW) collected by bronchoscopy will be analyzed for inflammatory cell counts (cells/ml).
Time frame: 6 hours
Oxylipin Metabolomic profiling
Metabolomic testing done with mass spectrometry on blood samples, BW and BAL.
Time frame: Baseline, 2 hours, 6 hours, 24 hours
Inflammation in bronchoalveolar lavage (BAL)
BAL collected by bronchoscopy will be analyzed for inflammatory markers, such as cell counts (cells/ml).
Time frame: 6 hour
Inflammation in endomucosal biopsies
Biopsies collected by bronchoscopy will be analyzed for inflammatory markers, such as cell counts (cells/mm2).
Time frame: 6 hours
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