The purpose of this study was to evaluate differences between propofol postconditioning and sevoflurane anesthesia in the intracranial aneurysm surgery about antioxidant effect.
intracranial aneurysm surgery may lead to regional ischemic reperfusion injury. Propofol, as an antioxidative phenol, has been demonstrated that mitigate the brain I/R in rats. As for sevoflurane, some investigation indicated that sevoflurane is able to reduce oxidative stress in cell(except neuronal cell lines ) and rodent models, but the antioxidant effect did not found in human minor incision surgeries, furthermore, oxidative stress was raised in the major surgeries including orthopedic surgeries, hysterectomy, cholecystectomy, and thoracotomy
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
PREVENTION
Masking
DOUBLE
Enrollment
60
administrated Target Controlled Infusion(TCI) of propofol (Cp 1.2ug/ml) and decreased sevoflurane with a Bispectral index (BIS) value of 40-60 to maintain anesthesia after clamp removal
0.5%-2% sevoflurane with BIS 40-60
Evidences of Clinically Definite Oxidative Stress Confirmed by ELISA Kit
Evidences of clinically definite oxidative stress :Superoxide dismutase activity, Hydroxyl radical
Time frame: before induction,after clamping removal ,operation ending ,1,3,7days post surgery
Evidences of Clinically Definite Oxidative Stress Confirmed by ELISA
Evidences of clinically definite oxidative stress:8-isoprostane,as a reliable biomarkers of lipid peroxidation
Time frame: before induction, after clamping removal,operation ending,1,3,7days post surgery
Evidences of Clinically Definite Oxidative Stress:Micronuclei
Evidences of clinically definite oxidative stress:micronuclei confirmed by Cytokinesis-block Micronucleus Test
Time frame: before induction,clamping removal ,operation ending,1,3,7days post surgery
Evidences of Clinically Definite Oxidative Stress Confirmed by High Performance Liquid Chromatography
Evidences of clinically definite oxidative stress :α- tocopherol,γ- tocopherol which was used to assess the antioxidant defense.
Time frame: before induction,after clamping removal ,operation ending ,1,3,7days post surgery
Evidences of Clinically Definite Oxidative Stress: Nuclear Buds
Evidences of clinically definite oxidative stress:nuclear buds Confirmed by Cytokinesis-block Micronucleus Test
Time frame: before induction,clamping removal ,operation ending,1,3,7days post surgery
Evidences of Clinically Definite Oxidative Stress: Nucleoplasmic Bridges
Evidences of clinically definite oxidative stress: nucleoplasmic bridges confirmed by Cytokinesis-block Micronucleus Test
Time frame: before induction,clamping removal ,operation ending,1,3,7days post surgery
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Mini Mental State Examination (MMSE)
A questionnaires is used to assess the cognitive function of patients in clinical,the total range was 0-30,and 27-30 were considered as normal value,\<27 were considered as recognitive dysfunction.
Time frame: before induction,1,3,7days post surgery
Montreal Cognitive Assessment (MoCA)
A questionnaires is used to assess the cognitive function of patients in clinical,the total range was 0-30,and 27-30 were considered as normal value,\<27 were considered as recognitive dysfunction.
Time frame: before induction,1,3,7days post surgery