Sepsis induces hemostatic disorders due to the exessive or inappropriate activation of inflammation, which could lead either to hypercoagulability or hypocoagulability. It is currently not possible to determine the hemostatic status of a given patient. This instability of hemostatic system is not revealed by classical tests. Thus, a better characterization of hemostatic status could certainly improve patient care. This study aims at characterizing disorders of coagulation and fibrinolysis using "global" tests such as thrombin generation test or coagulolytic test. Furthermore, the association with biological markers of interest (such as microparticles, neutrophil elastase or histones) will be evaluated.
Study Type
INTERVENTIONAL
Allocation
NON_RANDOMIZED
Purpose
DIAGNOSTIC
Masking
NONE
Enrollment
50
additional blood sampling (volume: 18 mL)
Changes in endogenous thrombin potential as assessed by thrombin generation test
thrombin generation will be measured using CAT method (fluorescence) in plasma from patients within 48 hours. Endogenous thrombin potential is defined as the area under the thrombin generation curve and will be compared with values obtained in healthy subjects
Time frame: 48 hours
Changes in Thrombin peak as assessed by thrombin generation test
thrombin generation will be measured using CAT method (fluorescence) in plasma from patients within 48 hours. Thrombin peak is defined as the highest thrombin concentration derived from the thrombin generation curve and will be compared with values obtained in healthy subjects.
Time frame: 48 hours
Changes in clot lysis time as assessed by clot lysis assay
Clot lysis assay will, be performed in plasma from patients and will be compared with those obtained in healthy subjects.
Time frame: 48 hours
Correlation of neutrophil elastase with changes in endogenous thrombin potential
Neutrophil elastase will be measured in plasma from patients.
Time frame: 48 hours
Correlation of cell-derived microparticles with changes in endogenous thrombin potential
microparticles derived from leukocytes, erythrocytes, platelets and endothelial cells will be measured in plasma from patients by flow cytometry.
Time frame: 48 hours
Correlation of circulating histones with changes in endogenous thrombin potential
Circulating histones will be measured in plasma from patients
Time frame: 48 hours
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