Aerosol BCG Challenge Trial in Healthy UK Adults

Inclusion Criteria: * Healthy adult aged 18-50 years * Resident in or near Oxford (CCVTM or OUH) or Birmingham (NIHR-WTCRF) for the = duration of the trial period * Screening IGRA negative * Chest radiograph normal * No relevant findings in medical history or on physical examination * Allow the Investigators to discuss the individual's medical history with their GP * Use effective contraception (see below) for the duration of the trial period (females only) * Refrain from blood donation during the trial * Give written informed consent * Allow the Investigator to register volunteer details with a confidential database (The - Over-volunteering Protection Service) to prevent concurrent entry into clinical studies/trials * Able and willing (in the Investigator's opinion) to comply with all the trial requirements Exclusion Criteria: * Previously resident for more than 12 months concurrently in a tropical climate where significant non-tuberculous mycobacterial exposure is likely * Participation in another research trial involving receipt of an investigational product in the 30 days preceding enrolment, or planned use during the trial period * Prior vaccination with BCG or any candidate TB vaccine * Administration of immunoglobulins and/or any blood products within the three months preceding the planned trial challenge date * Clinically significant history of skin disorder, allergy, atopy, immunodeficiency (including HIV), cancer (except BCC or CIS), cardiovascular disease, gastrointestinal disease, liver disease, renal disease, endocrine disorder, neurological illness, psychiatric disorder, drug or alcohol abuse * Concurrent oral or systemic steroid medication or the concurrent use of other immunosuppressive agents * History of anaphylaxis to vaccination or any allergy likely to be exacerbated by any component of the trial agent, sedative drugs, or any local or general anaesthetic agents * Pregnancy, lactation or intention to become pregnant during trial period Any respiratory disease, including asthma * Current smoker * Clinically significant abnormality on screening chest radiograph * Clinically significant abnormality of spirometry * Any nasal, pharyngeal, or laryngeal finding which precludes bronchoscopy * Current use of any medication taken through the nasal or inhaled route including cocaine or other recreational drugs * Clinical, radiological, or laboratory evidence of current active TB disease * Past treatment for TB disease * Any clinically significant abnormality of screening blood or urine tests * Positive HBsAg, HCV or HIV antibodies * Any other significant disease, disorder, or finding, which, in the opinion of the Investigator, may either put the volunteer at risk, affect the volunteer's ability to participate in the trial or impair interpretation of the trial data