The Effect of Anesthetics on Oxidative Stress and Apoptosis Status in Children

First Affiliated Hospital, Sun Yat-Sen University47 enrolled

Overview

The purpose of this study is to determine whether Propofol and Sevoflurane affect the oxidative stress and apoptosis status in children undergoing hypospadias repair surgery.

Study Type

INTERVENTIONAL

Allocation

RANDOMIZED

Purpose

BASIC_SCIENCE

Masking

SINGLE

Enrollment

Conditions

Anesthesia, General

Interventions

SevofluraneDRUG

PropofolDRUG

Eligibility

Sex: MALEMin age: 1 YearMax age: 3 Years

Medical Language ↔ Plain English

Inclusion Criteria: * American Society of Anesthesiology (ASA) I-II, undergoing selective surgery for hypospadias repair. Exclusion Criteria: * a history of developmental delay or mental retardation, which could make observational pain intensity assessment outside the norm; * a history of central nervous system diseases; * a known or suspected coagulopathy; * a known allergy to any of the study drugs; * abnormalities of the sacrum and any signs of infection at the site of the proposed caudal block.

Locations (1)

The First Affiliated Hospital of Sun Yat-Sen University

Guangzhou, Guangdong, China

Outcomes

Primary Outcomes

the change of blood glutathione peroxidase(GPx) level in perioperative period

Time frame: immediately after anesthetic induction, 2h and 3 days after operation

the change of blood-separated erythrocytes superoxide dismutase (SOD) activities in perioperative period

the blood sample was separated and erythrocytes was obtained, superoxide dismutase activity was measured using a commercially available assay-Ransod kit (Randox Laboratories, UK).

Time frame: immediately after anesthetic induction, 2h and 3 days after operation

the change of blood-separated erythrocytes catalase (CAT) activities in perioperative period

the blood sample was separated and erythrocytes was obtained, catalase activity was measured using the OxiSelect catalase activity assay (Cell Biolabs, San Diego, CA, USA)

Time frame: immediately after anesthetic induction, 2h and 3 days after operation

the change of blood-separated lymphocytes caspase-3 mRNA level in perioperative period

Lymphocytes were separated from whole blood and freshly used for real-time PCR of caspase-3

Time frame: immediately after anesthetic induction, 2h and 3 days after operation

Data from ClinicalTrials.gov

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