Effect of Frequent Interruptions of Prolonged Sitting on Self-perceived Levels of Energy, Mood, Food Cravings and Cognitive Function

Inclusion Criteria: 1. All ethnic groups and both genders 2. Age: Lower age limit: 19 years; Upper age limit: 45 years 3. Body-mass Index: Lower BMI limit: 18.5 kg/m2; Upper BMI limit: 29.9 kg/m2 4. Willing to adhere to caffeine restrictions of no more than two 16 ounce beverages in the morning of each study day with no caffeine intake after 12:00pm on any of the three study days Exclusion Criteria: 1. History of cardiovascular disease, including coronary artery disease, congestive heart failure, \& unstable angina, or uncontrolled hypertension (\>140/90 mm Hg) 2. Currently smoking or stopped smoking in the last 6 months 3. Medications affecting weight, energy intake, or energy expenditure in the last 6 months 4. Use of oral steroids 5. History of stroke or seizures, thyroid disease, type 1 or 2 diabetes, Cushing's syndrome, cerebrovascular, renal disease, hepatic disease, arrhythmias 6. Cancer requiring treatment in the past 5 years, with the exception of skin cancers other than melanoma 7. Infectious diseases: Self-reported HIV positivity or active tuberculosis 8. Inability to exercise for more than 30 minutes on a treadmill at moderate intensity 9. Currently pregnant, lactating or less than 6 months post-partum 10. History of a major psychiatric disorder including schizophrenia, bipolar illness or psychotic depression 11. Score of \>25 on Beck Depression Inventory 12. No recreational drug use including marijuana in the past 1 year