The aim of the study is to understand more about how different fatty acids modulate postprandial lipid metabolism and inflammatory response.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
PREVENTION
Masking
QUADRUPLE
Enrollment
31
Muffin rich in saturated fat.
Muffin rich in polyunsaturated fat.
University of Oslo
Oslo, Post Box 1046, Blindern, Norway
Change in levels of circulating triglycerides
Time frame: Measured at baseline and 2,4 and 6 hours after intake of test meal
Changes in markers of lipid- and glucose metabolism
Changes in levels of total cholesterol, low-density lipoprotein, high-density lipoprotein, apolipoprotein (apo) B, apo A1, apo CIII, apo B48, lipoprotein (a), free fatty acids, total fatty acid composition, LDL-receptor-related protein with 11 ligand-binding repeats (LR11), HbA1c, glucose, insulin and troponin.
Time frame: Measured at baseline and 2,4 and 6 hours after intake of test meal
Changes in circulating levels of inflammatory markers
Changes in levels of inflammatory markers in circulation such as i.e. cytokines and hsCRP
Time frame: Measured at baseline and 2,4 and 6 hours after intake of test meal
Changes in PBMC gene expression levels of markers of inflammation and lipid metabolism
Changes in levels of markers of inflammation and lipid metabolism at PBMC gene expression level
Time frame: Measured at baseline and 2, 4 and 6 hours after intake of test meal
Changes in lipid classes and lipoprotein size
Time frame: Measured at baseline and 2,4 and 6 hours after intake of test meal
Changes in plasma and urine metabolomics
Changes in metabolites such as glucose, lactate, pyruvate, citrate and amino acids will be measured in plasma and urine.
Time frame: Measured in plasma at baseline and 2,4 and 6 hours after intake of test meal. Measured in urine at fasting state and during the 6 hour postprandial phase.
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Check DNA for single nuclear polymorphisms
Time frame: Measured at baseline and 2,4 and 6 hours after intake of test meal
Changes in PBMC Whole genome transcriptomics
Time frame: Measured at baseline and 4 and 6 hours after intake of test meal