In this study, the aim is to identify the modifications responsible for aseptic osteonecrosis of the femoral head and its structural evolution by the association of the micro scanner analysis and Raman spectrometry performed on the femoral heads removed during hip replacements. The study of femoral heads will allow the analysis of bone tissue at two different scales, both correlated with the biomechanical properties of the bone. Also, the association with preliminary MRI analysis will provide pathogenic explanations correlated to these modifications.
Study Type
INTERVENTIONAL
Allocation
NON_RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
NONE
Enrollment
34
Hospital Salengro CHU Lille
Lille, France
Hospital Group of the Catholic Institute of Lille
Lomme, France
Osseous modifications responsible of osteonecrosis
Time frame: through the study completion, an average of 18 months
Total cross-sectional area
by using a contrast-enhanced nano-CT for the subchondral plate in necrotic zone, juxta-necrotic zone, remotely of the necrotic zone and cortical of the femoral collar
Time frame: through the study completion, an average of 27 months
cortical bone area
by using a contrast-enhanced nano-CT for the subchondral plate in necrotic zone, juxta-necrotic zone, remotely of the necrotic zone and cortical of the femoral collar
Time frame: through the study completion, an average of 27 months
cortical area fraction
by using a contrast-enhanced nano-CT for the subchondral plate in necrotic zone, juxta-necrotic zone, remotely of the necrotic zone and cortical of the femoral collar
Time frame: through the study completion, an average of 27 months
cortical thickness
by using a contrast-enhanced nano-CT for the subchondral plate in necrotic zone, juxta-necrotic zone, remotely of the necrotic zone and cortical of the femoral collar
Time frame: through the study completion, an average of 27 months
bone volume fraction
by using a contrast-enhanced nano-CT for the subchondral trabecular bone
Time frame: through the study completion, an average of 27 months
trabecular number
by using a contrast-enhanced nano-CT for the subchondral trabecular bone
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Time frame: through the study completion, an average of 27 months
trabecular separation
by using a nano-CT for the subchondral trabecular bone
Time frame: through the study completion, an average of 27 months
trabecular thickness
by using a contrast-enhanced nano-CT for the subchondral trabecular bone
Time frame: through the study completion, an average of 27 months
Physico-chemical composition of the trabecular subchondral bone
mineralisation, carbonation, crystallinity, secondary structure of collagen, maturity of collagen, relative content of proteoglycans. These measures will be done for the trabecular subchondral bone in necrotic zone, juxta-necrotic zone, remotely of the necrotic zone and in the cortical of the femoral collar
Time frame: through the study completion, an average of 27 months
Existence and extent of edema
this measure will be done on the T2-weighted sequences with fat suppression
Time frame: through the study completion, an average of 27 months
Perfusion MRI sequence
morphological aspect of the enhancement curve, semi-quantitative parameters (slope, area under the curve, time to peak) and pharmacokinetic parameters
Time frame: through the study completion, an average of 27 months
Medullary fat fraction in T1-weighted DIXON method
border of osteosclerosis, identification of a necrotic zone, extent of this zone
Time frame: through the study completion, an average of 27 months
Evaluation of osteocyte and adipocyte viability in hematoxylin-eosin-saffron (HES) staining
Time frame: through the study completion, an average of 27 months
Number and ramifications of sub-chondral microvessels by immuno-labeling with CD21
Time frame: through the study completion, an average of 27 months
Evaluation of the number of osteoclasts by TRAP (tartrate acid Phosphatase) staining
Time frame: through the study completion, an average of 27 months
Mankin score for cartilage degradation by Safranin O staining
Time frame: through the study completion, an average of 27 months