Trial Ruxolitinib and Peg-interferon Alpha-2a Combination in Patients With Primary Myelofibrosis RUXOPeg

Inclusion Criteria: Patients eligible for inclusion in this study have to meet all of the following criteria: * Age \> 18 years and \< 66 years * Diagnosis of primary or secondary myelofibrosis according to the 2008 World Health Organization (WHO) criteria for PMF (Tefferi and Vardiman 2008) and the proposed criteria for PPV-MF and PET-MF outlined by the International Working Group for Myelofibrosis Research and Treatment (Barosi et al 2008) * Patients classified as high risk, OR intermediate risk-2, OR intermediate risk-1, as defined by the International Working Group, IWG (Cervantes, et al 2009) at diagnosis (or by the DIPSS (Passamonti et al. 2010) for patients assessed after diagnosis of PMF) * Need for active therapy, defined as presence of at least one of the following: * symptomatic splenomegaly * presence of constitutional symptoms * anemia (Hb\< 10g/dl) * leukocytosis \> 25 G/l * thrombocytosis \> 400 G/l * Presence of JAK2V617F , Calreticulin or MPL mutations * Platelet counts ≥ 150 x 109/L not reached with the aide of transfusions at screening * Patients with ANC ≥ 1.5 x 109/L at screening without the use of G-CSF * Peripheral blood blast count of ≤ 10% at Screening * ECOG performance status of 0, 1, or 2 at Screening * Negative serological Hepatitis B test (i.e. HBsAg negative and anti-HBc negative, patients positive for anti-HBc may be included if PCR for HBV DNA is negative); negative testing of Hepatitis C RNA; negative HIV test within 6 weeks prior to registration. * Women of childbearing potential must have a negative serum (beta-human chorionic gonadotropin \[beta-hCG\]) or urine pregnancy test at Screening and effective contraception's method during the study and 75 days after the last dose. * Informed consent sign * Patients without Social Security coverage * Patient who is not included into another clinical study until 1 month after the end of this study Exclusion Criteria: * ANC \< 1.5 G/l or platelets \< 150 G/l * \> 10% circulating blasts * Contra-indication to IFN alpha or to ruxolitinib * Patients previously treated with IFN alpha or a JAK2 inhibitor * Documented autoimmune disease at screening or in the medical history * History or presence of depression requiring treatment with antidepressant * Evidence of severe retinopathy (e.g. cytomegalovirus retinitis, macular degeneration) or clinically relevant ophthalmological disorder (due to diabetes mellitus or hypertension) * Thyroid dysfunction not adequately controlled * Women of childbearing potential who have a positive serum pregnancy test at screening or who cannot or do not wish to use an effective method of contraception, during treatment and for 75 days after the last dose of study drug. * Pregnant or nursing (lactating) women * Patients with known active hepatitis B or C or with known HIV positivity * Patient with a concurrent malignancy or malignancy within 3 years of Screening, with the exception of adequately treated basal or squamous cell carcinoma, non melanomatous skin cancer or curatively resected cervical cancer. * Patient has a history of cardiac dysfunction including any of the following: * Myocardial infarction documented by elevated cardiac enzymes or persistent regional wall abnormalities on assessment of LVEF function * History of documented congestive heart failure (New York Heart Association functional classification III-IV) * Documented cardiomyopathy * Patient has active cardiac disease including any of the following: * Left Ventricular Ejection Fraction (LVEF) \< 50% as determined by Multiple Gated Acquisition (MUGA) or echocardiogram (ECHO) * QTc\> 480 msec on screening ECG (QTcF, using the Fridericia formula) * Angina pectoris that requires the use of anti-anginal medication * Ventricular arrhythmias except for benign premature ventricular contractions * Supraventricular and nodal arrhythmias requiring a pacemaker or not controlled with medication * Conduction abnormality requiring a pacemaker * Valvular disease with documented compromise in cardiac function * Symptomatic pericarditis * Patients with inadequate liver or renal function at Screening as demonstrated by: * encephalopathy grade 1 or more, as per West Haven Criteria * known hepatocellular disease (e.g. active hepatitis or cirrhosis) * total bilirubin\> 2 x ULN and subsequent determination of direct bilirubin \> 2 x ULN alanine aminotransferase (ALT) and aspartate aminotransferase (AST) \> 2 x ULN * MDRD-eGFR\< 45 mL/min/1.73m2 or on dialysis * Patients who currently are willing candidates for a stem cell transplantation at the time of the screening assessments