Influenza Challenge Study in Healthy Volunteers

Inclusion Criteria: * Age 18 to 45 years, inclusive. * Comprehension of the study requirements;availability for the required study period, ability to attend scheduled study visits, and willingness to participate in the inpatient challenge. * Willingness to provide written consent for participation after reading the Consent Form and after having adequate opportunity to discuss the study with an investigator or qualified deputy. * Good general health status as determined by a screening evaluation no greater than 45 days prior to the quarantine and challenge phase. * For female subjects, provision of a history of reliable contraceptive practices \[hysterectomy or bilateral tubal ligation, oral or implanted contraceptive use, intrauterine device, barrier method plus spermicide, history of a single male partner with vasectomy, or a history of abstinence deemed credible by the investigator(s)\]. The provision of this history does NOT replace the requirement to perform, and obtain negative results in, pregnancy tests. Exclusion Criteria: * Presence of any significant acute or chronic, uncontrolled medical or psychiatric illness, * Health care workers (including doctors, nurses, medical students and allied healthcare professionals) anticipated to have patient contact within two weeks of viral challenge. Healthcare workers who volunteer should not work with patients until 14 days after challenge or until their symptoms are fully resolved. * Venous access deemed inadequate for the phlebotomy demands of the study. * Positive serologic tests for HIV, Hepatitis B surface antigen, or Hepatitis C antibody. * Evidence of drug abuse or a positive urine Class A drug or alcohol screen. * Female subjects, who are known to be pregnant or who have a positive urine β-HCG detected prior to challenge. * Chronic use of any medication or other product (prescription or over-the-counter), for symptoms of rhinitis or nasal congestion or for any chronic nasopharyngeal complaint, or chronic use of any intranasal medication for any indication. * Any history during adulthood of asthma of any etiology (chronic obstructive pulmonary disease), or any use of a bronchodilator or other asthma medication within adulthood. * Smokers unwilling/unable to desist for the quarantine phase duration of the study. * Subject is type I or type II diabetic. * Subject is allergic to Gentamicin. * Acute use of any medication or other product, prescription or over-the-counter, for symptoms of rhinitis or nasal congestion within seven (7) days prior to challenge. This includes any corticosteroid or beta agonist containing nasal spray. * An Abnormal ECG deemed clinically relevant by the Investigator(s). * Any anatomic or neurologic abnormality impairing the gag reflex or conducive to aspiration, or history suggestive of such a problem or any abnormality significantly altering the anatomy of the nose or nasopharynx. * Receipt of systemic glucocorticoids (in a dose ≥5 mg prednisone daily or equivalent) within one month, or any other cytotoxic or immunosuppressive drug within six months prior to challenge. * Receipt of any investigational drug within six (6) months prior to challenge, or prior participation in a clinical trial of any Influenza vaccine, or any investigational vaccine or experimental Influenza viral challenge delivered directly to the respiratory tract within 1 year prior to challenge. * Presence of any febrile illness or symptoms of upper viral respiratory infection: 1. existing on the day of challenge or between admission for Influenza challenge and administration of the challenge inoculum. (Such subjects may be re-evaluated for enrollment after resolution of the illness); 2. within two (2) weeks prior to challenge or if challenge is set to occur during November, December, January, February, or March if there are any symptoms suggestive of viral respiratory infection occurring between screening and challenge. * History of frequent epistaxis (nose bleeds). * Presence of household member or close contact (for an additional two weeks after discharge from the isolation facility) who is: (a) less than 3 years of age; (b) any person with any known immunodeficiency; (c) any person receiving immunosuppressant medications; (d) any person undergoing or soon to undergo cancer chemotherapy within 28 days of challenge; (e) any person who has diagnosed emphysema or COPD, is elderly residing in a nursing home, or with severe lung disease or medical condition including but not exclusive to the conditions listed in Appendix 8; or (f) any person who has received a transplant (bone marrow or solid organ). * Any laboratory test which is abnormal and which is deemed by the Investigator(s) to be clinically significant. (This includes blood chemistry, haematology, cardiac iso-enzymes, or urinalysis). * Known IgA deficiency, immotile cilia syndrome, or Kartagener's syndrome. * History of seasonal hay fever or a seasonal allergic rhinitis (SAR), including the use of symptomatic prescription only medication and non prescription medication. * As a result of the medical interview, physical exam, or screening investigations, the Investigator(s) considers the subject unfit for the study. * Those employed or immediate relatives of those employed at Retroscreen Virology or the study site. * Receipt of a northern hemisphere seasonal influenza vaccine in the 2006/07/08 winter seasons. * Receipt of any systemic chemotherapy agent at any time.