The Effect of Bitter Taste Receptor Agonists on The Gastrointestinal Tract, Hunger and Food Intake

Universitaire Ziekenhuizen KU Leuven98 enrolled

Overview

In this study, the investigators aimed at evaluating the role of bitter taste receptors in the gastrointestinal tract (GIT). Intragastric or intraduodenal administration of denatonium benzoate (DB) or quinine hydrochloride were compared with placebo administration for their effects on lingual sensitivity, gastrointestinal motility (both in the fasted and fed state), gut hormone release (motilin, ghrelin, glucagon-like peptide-1 (GLP-1) and cholecystokinin (CCK)) and food intake. Differences between lean and obese subjects will be evaluated.

Study Type

INTERVENTIONAL

Allocation

RANDOMIZED

Purpose

BASIC_SCIENCE

Masking

SINGLE

Enrollment

Conditions

Healthy Obesity

Interventions

Eligibility

Sex: ALLMin age: 18 YearsMax age: 60 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * BMI\>30 kg/m² for the obese volunteers * BMI\<30 kg/m² for the lean volunteers * Subject is capable and willing to give informed consent * Female volunteers of child bearing potential must use oral, injected or implanted hormonal methods of contraception Exclusion Criteria: * Female volunteer is pregnant or breastfeeding * Gastrointestinal diseases, major abdominal surgery * Major psychiatric illnesses * Volunteers that use drugs affecting the GIT or the central nervous system (CNS) * Volunteers that suffer from diabetes mellitus * Volunteers suffering from an endocrine disease such as diabetes, Cushing's disease, Addison's disease, hypothalamic tumor… * Volunteers that have undergone surgical procedure for weight loss

Outcomes

Primary Outcomes

Change in gastrointestinal motility measured by antroduodenal high-resolution manometry

Time frame: 2 hours after administration, continuous measurement with high resolution manometry

Secondary Outcomes

Change in gut hormone release measured by specific radioactive immunoassays

Time frame: 2 hours after administration, blood sample every 10 min

Change in food intake measured by the caloric content of the meal

Time frame: ad libitum food intake for 1 hour, 40 min after administration

Change in subjective hunger and satiety scores measured by visual analogue scales of 100 mm

Time frame: 2 hours after administration, assessment every 5 min