Objective: In this study the investigators aim at investigating: 1. probiotics ability to modulate the microbiome and microbial translocation, 2. if probiotics affect the level of cholesterol, triglycerides as markers of cardiovascular risk factors and 3. if a reduction of microbial translocation is associated with a reduction of inflammation in the gastro-intestinal tract. Design: The study is a prospective clinical intervention trial of 40 HIV-infected patients. Method: The investigator will administer the bacteria Lactobacillus Rhamnosus in capsular form to each patient 2 times a day in 8 weeks. At baseline and at the 8th week of the intervention, the investigators will collect blood samples, feces samples and make a positron emission tomography-magnetic resonance scans.
Study Type
INTERVENTIONAL
Allocation
NA
Purpose
PREVENTION
Masking
NONE
Enrollment
45
The probiotic strain Lactobacillus rhamnosus will be self-administrated twice a day, one capsule in the morning and one in the evening for eight weeks.
Rigshospitalet
Copenhagen, Denmark
change in Lipopolysaccharide (unit= endotoxin unit/mL) from baseline after intervention
will be measured in plasma samples
Time frame: Will be measured before and after eight weeks of intervention.
change in soluble cluster of differentiation 14 (unit (ng/mL) from baseline and eight weeks after intervention.
will be measured in plasma samples
Time frame: Will be measured before and after eight weeks of intervention.
change in inflammation around the gut measured with positron emission tomography-magnetic resonance scans of the abdomen ( size of the lymph nodes and positron emission tomography-activity)
positron emission tomography-magnetic resonance scans of the abdomen before an after intervention
Time frame: Will be measured before and after eight weeks of intervention.
Changes in of high sensitive C-reactive protein (unit mg/l) from baseline and after eight weeks of intervention
will be measured in plasma samples
Time frame: Will be measured before and after eight weeks of intervention.
Changes in measures of cytokines unit (pg/ml) at baseline and after eight weeks of intervention
will be measured in plasma samples
Time frame: Will be measured before and after eight weeks of intervention.
Changes in the gut microbiota composition (454 pyrosequencing of fecal samples) from baseline to eight weeks after intervention (end of study)
stool-samples will be collected an analyzed by deep sequencing
Time frame: Will be measured before and after eight weeks of intervention.
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