Nowadays, an increase of inflammatory chronic diseases and/or tumors incidence has been reported in part due to the rising in life expectancy. For instance, exposure to air pollution, and in particularly to fine particles has been classified by the International Agency for Research on Cancer (IARC) as a risk factor for cancer, being the elderly population particularly sensitive. However, no validated biomarkers have been identified to assess the exposure to fine particles maybe correlated to cancerogenesis. AEROTOX-2 is a prospective pilot study on the ex vivo effects of the atmospheric pollution exposure. The study aims to identity new biomarkers after an exposure of leukocytes to doses of fine particles. Secondly, the study aims to analyze, according to age, the leukocytes response to the urban pollution exposure.
To estimate the influence of the atmospheric pollution on the whole adult population, subjects will be recruited in a wide range of age (20 - 80 years). Samples of atmospheric particles will be collected in Dunkerque (North of France), one of the most important industrial areas in France. This city is influenced by car traffic, urban and industrial activities, and the wide sea traffic of the North Sea. Therefore, Dunkerque is a reference site to study the atmospheric pollution and its impact on health. These markers will be searched in association with the different mechanisms involved in the toxicity of the fine particles such as: inflammatory response, genotoxicity, metabolic activity and epigenetic changes after exposure of leukocytes isolated from patients to doses of fine particles. The study of the correlation between biological observations and conditions of exposure to air particles will bring answers about the impact of the environmental pollution on the health of vulnerable populations.
Study Type
INTERVENTIONAL
Allocation
NON_RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
NONE
Enrollment
101
Clinical Nutrition Center Naturalpha
Lille, Hauts-de-France, France
Level of expression of blood biomarkers of air pollution exposure
These markers will be searched in association with the different mechanisms involved in the toxicity of the fine particles such as: inflammatory response, genotoxicity, metabolic activity and epigenetic changes after exposure of leukocytes to doses of fine particles.
Time frame: through the study completion, an average of 48 months
Total amount of different populations of peripheral blood leucocytes
After exposure of leukocytes to doses of fine particles.
Time frame: through the study completion, an average of 48 months
Measure of the expression of genes involved in metabolism by quantitative polymerase chain reaction (qPCR)
After exposure of leukocytes to doses of fine particles.
Time frame: through the study completion, an average of 48 months
Measure of the expression of genes involved in metabolism by TaqMan Gene Expression Assays
After exposure of leukocytes to doses of fine particles.
Time frame: through the study completion, an average of 48 months
Detection of genotoxicity by identification of DNA double-strand breaks and DNA adducts
Time frame: through the study completion, an average of 48 months
Detection of epigenetic modifications
After exposure of leukocytes to doses of fine particles. In order to measure the expression of hundreds of miRNAs within a given sample a microarray analysis technology will be performed using the TaqMan® Low Density Array Human MicroRNA.
Time frame: through the study completion, an average of 48 months
Correlation between age and markers of inflammation
After exposure of leukocytes to doses of fine particles.
Time frame: through the study completion, an average of 48 months
Correlation between age and markers of metabolic activity
After exposure of leukocytes to doses of fine particles.
Time frame: through the study completion, an average of 48 months
Correlation between age and markers genotoxicity
After exposure of leukocytes to doses of fine particles.
Time frame: through the study completion, an average of 48 months
Correlation between age and markers of epigenetic modifications
After exposure of leukocytes to doses of fine particles.
Time frame: through the study completion, an average of 48 months
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