Bioavailability and Bioactivity of Red Wine Melatonin in Humans

University of Milan12 enrolled

Overview

Melatonin (MLT) is a bioactive compound in red wine. The investigators will investigate the serum and salivary concentrations and antioxidant power of MLT after the intake of a glass of red wine. Twelve healthy volunteers were recruited to receive both 125 mL of red wine rich in MLT (10 ng mL-1) and without MLT (placebo), via a randomized, cross-over, and double-blind design. Serum and salivary MLT will be measured by liquid chromatography coupled to mass spectrometry (LC-MS) and enzyme-linked immunosorbent (ELISA), respectively. The ferric ion reducing antioxidant power (FRAP) will be analyzed.

Melatonin (MLT) is a bioactive compound in red wine. No information is currently available on serum and salivary concentrations of MLT after drinking a glass of red wine, containing this indoleamine. Hence, in this study, the investigators will investigate the levels of MLT, in serum and saliva, soon after the intake of the MLT-rich red wine, and, correspondingly, they will verify the antioxidant power of the same biological fluids. This will be a randomized, crossover, double-blind, placebo-controlled study with two treatment arms. Twelve healthy volunteers will be recruited to receive both 125 mL of red wine rich in MLT (10 ng mL-1) and without MLT (placebo), via a randomized, cross-over, and double-blind design. Serum and salivary MLT will be measured by liquid chromatography coupled to mass spectrometry (LC-MS) and enzyme-linked immunosorbent (ELISA), respectively. The ferric ion reducing antioxidant power (FRAP) will be also analyzed. For serum analyses, samples will be collected at 0, 30, 60 and 90 min. For saliva analyses, samples will be collected at 0, 45 and 120 min.

Study Type

INTERVENTIONAL

Allocation

RANDOMIZED

Masking

QUADRUPLE

Enrollment

Conditions

Bioavailability

Interventions

Red wine with melatoninOTHER

Twelve healthy volunteers will be recruited to receive both 125 mL of red wine rich in MLT (10 ng mL-1)

Red wine without melatoninOTHER

Twelve healthy volunteers will be recruited to receive both 125 mL of red wine without MLT

Eligibility

Sex: ALLMin age: 20 YearsMax age: 45 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * to be healthy * to have normal weight (BMI 18.5-25.0) * to voluntarily accept to participate to the study. Exclusion Criteria: * pregnancy and lactation for women; * supplementation with MLT and/or any other dietary supplement (vitamins, antioxidants, botanicals, phytochemicals) * systemic and chronic-degenerative diseases * abnormal hematological parameters * heavy smoking and alcohol drinking * high-intensity physical activity * under pharmacological treatment

Locations (1)

University of Milan

Milan, Italy, Italy

Outcomes

Primary Outcomes

Serum and salivary melatonin after red wine intake (with or without MLT)

LC-MS analyses for serum and ELISA analyses for saliva

Time frame: Time-course of the serum melatonin levels in healthy volunteer from 0 min to 90 min after administration of red wine. Time-course of the salivary melatonin levels in healthy volunteer from 0 min to 120 min after administration of red wine.

Secondary Outcomes

Antioxidant power of saliva and serum after red wine intake (with or without MLT)

FRAP analyses

Time frame: Time-course of the serum antioxidant power in healthy volunteer from 0 min to 90 min after administration of red wine. Time-course of the salivary antioxidant power in healthy volunteer from 0 min to 120 min after administration of red wine.

Data from ClinicalTrials.gov

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