The purpose of this study is to determine in situ the bacterial viability, thickness and bacterial diversity of a biofilm formed on different substrates in comparison to a naturally tooth-formed oral biofilm. In addition, it will be evaluated whether the restraint of oral hygiene measures may influence the development of the PL-Biofilm.
* To calculate an a priori sample size, the following statistical criteria were established: an effect size of 0.35, an alpha error of 0.05 and a statistical power of 87%. Assuming these criteria and using the repeated measures ANOVA test, a sample size of 15 subjects was required. * Bacterial viability and thickness will be analyse by Confocal Laser Scanning Microscope and Live/Dead BackLight staining solution. * Bacterial diversity will be analyse by Pyrosequencing the 16S DNAr gene.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
DOUBLE
Enrollment
15
Normal toothbrushing without toothpaste or antiseptic mouthwash.
Univeristy of Santiago de Compostela, Faculty of Dentistry
Santiago de Compostela, Galicia/ A Coruña, Spain
RECRUITINGBacterial viability
It will be measured by analysis of images taken by Confocal laser Scanning Microscope
Time frame: Bacterial viability measured in proportion of viable/no viable bacteria after 48 hours of exposition to oral cavity
Bacterial diversity
It will be measured by 16S rDNA gene pyrosequencing and posterior bioinformatic analysis.
Time frame: Bacterial diversity measured by microbial estimators of abundance and richness after 48 hours of exposition to oral cavity
Biofilm thickness
It will be measured by analysing sectional images taken by Confocal Laser Scanning Microscope.
Time frame: Oral biofilm thickness measured by analysis of sectional images after 48 hours of exposition to oral cavity
This platform is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional.