Impact of Once-Weekly Rifapentine and Isoniazid on the Steady State Pharmacokinetics of Dolutegravir and Darunavir Boosted With Cobicistat in Healthy Volunteers

* INCLUSION CRITERIA: A subject will be considered eligible for this study only if all of the following criteria are met: 1. Ages 18 - 65 years 2. Weight greater than or equal to 45 kg and less than or equal to 120; BMI greater than or equal to 18.0 and \<30 3. Judged to be healthy based on medical history, physical examination, vital signs, and clinical laboratory tests (liver function tests (AST, ALT, Tbili) greater than or equal to upper limit of normal \[ULN\], serum creatinine (SCr) less than or equal to ULN, CK less than or equal to 2X ULN, platelets (PLT) \>150,0000/mm3, hemoglobin (Hgb) \>11 g/dL), C-reactive protein (CRP) less than or equal to ULN) 4. Negative QuantiFERON-TB Gold test at screening 5. HIV-negative, as determined by standard serologic assays for HIV infection. 6. No laboratory evidence of active Hepatitis A, B, or C infection 7. Willing to abstain from alcohol consumption throughout the study period 8. Subject agrees to genetic testing and storage of specimens for future research 9. Negative serum or urine pregnancy test for females of child-bearing potential 10. For female subjects able to become pregnant (i.e., have not undergone surgical sterilization or are not postmenopausal), willingness to prevent pregnancy during the study period by: 1. Practicing absolute abstinence from sexual contact or 2. Committing to use of effective non-hormonal and/or barrier methods of birth control during any and all sexual encounters. Acceptable methods are as follows: * Condom, diaphragm, or cervical cap with a spermicide * Intrauterine device (IUD) without hormones * Male partner with a vasectomy EXCLUSION CRITERIA: A subject will be ineligible for this study if 1, or more, of the following criteria are met: 1. Known hypersensitivity to dolutegravir, darunavir, cobicistat, rifapentine and other rifamycin analogues, or isoniazid 2. History of type 1 hypersensitivity reaction to sulfonamides 3. History or presence of any of the following: 1. Latent or active TB infection 2. Gastrointestinal disease that is uncontrolled, requires daily treatment with medication, or would interfere with a subject s ability to absorb drugs (diarrhea, pancreatitis, peptic ulcer disease, etc.), 3. Renal impairment (chronic renal insufficiency of any CKD stage, or acute renal failure not induced by drug therapy defined as GFR \< 90 ml/min) 4. Respiratory disease that is uncontrolled or requires daily treatment with medication (asthma, chronic obstructive pulmonary disease, etc.) 5. Cardiovascular disease (hypertension \[systolic blood pressure \>140 mmHg or diastolic blood pressure \> 90 mmHg\], heart failure, arrhythmia, etc.) 6. Metabolic disorders (diabetes mellitus, etc.) 7. Hematologic or bleeding disorders (anemia, hemophilia, serious/major bleeding events, menorrhagia (female subjects), etc.) 8. Immunologic disorders 9. Hormonal or endocrine disorders 10. Psychiatric illness that would interfere with his or her ability to comply with study procedures or that requires daily treatment with medication 11. Seizure disorder, with the exception of childhood febrile seizures 12. Malignancy, or 13. Any other condition that may interfere with the interpretation of the study results, or not be in the best interest of the subject in the opinion of the investigator 4. Fasting total cholesterol \>240 mg/dL or fasting triglycerides \>240 mg/dL on 2 consecutive visits 5. Fasting glucose \>125 mg/dL on 2 consecutive visits 6. Current participation in an onging investigational drug protocol or use of any investigational drug within 30 days (based on last dose received) prior to receipt of any study drugs/medications. 7. Therapy with any prescription, over-the-counter, herbal, or holistic medications, including hormonal contraceptives by any route, within 5 half-lives of the agent prior to receipt of any study medications will not be permitted with the following exception: Intermittent or short-course therapy (\< 14 days) with prescription or over-the-counter medications, herbals, or holistic medications within the screening period prior to starting study drug may be permitted, and will be reviewed by investigators on a case-by-case basis for potential drug interactions. Receipt of influenza vaccination will be allowed prior to, during, and/or after the study 8. Inability to obtain venous access for sample collection 9. Inability to swallow whole capsules and/or tablets 10. Current breastfeeding 11. Drug or alcohol use that may impair safety or adherence 12. Use of nicotine-containing products, including cigarettes and chewing tobacco, nicotine patches, gum, electronic cigarettes, etc. 13. Organ or stem cell transplant recipient 14. Uncorrected and persistent electrolyte abnormalities (e.g., potassium, magnesium, and calcium) 15. Current alcohol abuse or alcohol dependence disorders (DSM-5 criteria)