Culture media used to support development of zygotes to the blastocyst stage is based on two basic philosophies: a single step medium "let-the-embryo-choose" or a sequential medium "back-to-nature". For single step media, there are two types of protocol can be used for the culture of human preimplantation embryos: 1. Interrupted single medium culture, in which the medium is renewed on the Day 3 of embryo culture 2. Uninterrupted single embryo culture, in which the same single medium is used throughout the 5-6 days of culture with no replenishment or replacement on Day 3. Investigators aim to compare the blastocyst development and clinical outcome between the two types of protocols of a single medium (with or without renewal on Day 3)
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Masking
DOUBLE
Enrollment
200
Sunrise fertility center
Cairo, Egypt
RECRUITINGClinical pregnancy rate
diagnosed by the presence of a heartbeat in the gestational sac visualized by ultrasound
Time frame: 4 weeks post embryo transfer
Implantation rate
the number of intrauterine gestational sacs over the total number of embryos transferred
Time frame: 4-6 weeks post embryo transfer
Blastocyst formation rate
Time frame: 5-6 days post intracytoplasmic sperm injection
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