Predictive biomarkers are needed to identify those patients with higher risk of recurrence after surgery for colon cancer with curative intent. Our main objective is to determine a metabolite profile in blood plasma from patients operated from colorectal cancer that can be associated with the oncologic outcome and be validated as predictive biomarkers in future studies. A secondary objective is to study the glycolytic metabolism of colon cancer cell lines treated with plasma samples from the same patients. In particular, to validate the increased utilization of lactate by tumor cells as a metabolic substrate using postoperative human samples. Patients with colorectal cancer that have undergone surgical resection will be included. Plasma samples will be obtained before surgery and the 4th day and the 3rd, 6th, 12th, and 18th months after surgery. Metabolic profiles in plasma samples will be determined using a kit that allows the quantification of 180 metabolites by mass spectrometry. A clinical follow up will be maintained for at least 2 years to identify tumor recurrences.
Up to 30-40% of patients operated from colorectal cancer display tumor recurrence. Predictive biomarkers are needed to identify those patients with higher risk of recurrence. Our main objective is to determine a metabolite profile in blood plasma from patients operated from colorectal cancer that can be associated with the oncologic outcome and be validated as prognostic biomarkers in future studies. A secondary objective is to study the glycolytic metabolism of colon cancer cell lines treated with plasma samples from the same patients. In particular, to validate the increased utilization of lactate by tumor cells as a metabolic substrate using postoperative human samples, as it was previously observed by us in vitro using an inflammatory environment in conditions of hypoxia and lack of glucose. Patients with colorectal cancer that have undergone surgical resection will be included. Plasma samples will be obtained before surgery and the 4th day and the 3rd, 6th, 12th, and 18th months after surgery. Metabolic profiles in plasma samples will be determined using a kit that allows the quantification of 180 metabolites by mass spectrometry. Cellular assays will be performed on the SW620 and HT-29 colon cancer cell lines. Cells will be treated with plasma samples and the concentration of lactate and other metabolites will be analyzed in the medium supernatants. A clinical follow up will be maintained for at least 2 years to identify tumor recurrences.
Study Type
OBSERVATIONAL
Enrollment
130
Segmental resection for colon cancer and anterior resection in patients with rectal cancer
Hospital del Mar Medical Research Institute
Barcelona, Barcelona, Spain
RECRUITINGDisease-free survival
Time from the date of surgery to the date of first documentation of recurrence
Time frame: 5 years from the date of surgery
Disease-specific survival
Time from the date of surgery to death by colon cancer
Time frame: 5 years from the date of surgery
Local recurrence
Tumor associated with surgical site (anastomosis, tumor bed, and mesentery) and confirmed histologically or by imaging.
Time frame: 5 years from the date of surgery
Systemic recurrence
Spread of the disease outside the surgical field to organs such as the liver, lungs, bones, or brain
Time frame: 5 years from the date of surgery
Postoperative intra-abdominal sepsis
Anastomotic leak or intra-abdominal abscess
Time frame: 30 days from the date of surgery
Postoperative mortality
Time frame: 30 days from the date of surgery
This platform is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional.