Type 2 diabetes is the predominant type of diabetes. Because a quiet evolution, it is difficult to have a rapid diagnose. A better knowledge about pathophysiological mechanisms at the origin of type 2 diabetes and about its complications could make it possible to improve the prevention and the treatment of this disease. Research team developped a new research axis : the microbiota of the intestinal mucosa. They proved a translocation process of intestinal bacteria from the intestinal mucosa to different tissue of the organism implicated in glucose homeostasis. This mechanism is involved in the type 2 diabetes development. A clinical study (MICIMAB) of predictive biomarkers of diabetes and obesity is ongoing. In parallel, the same team explored the role of intestinal immunity modifications in the bacteria translocation from the gut to the blood circulation. They already have some results on animal model but not yet in human. In animal model, a solid reduction of lymphocytes T CD4 Th17 in the intestinal wall is responsable of the translocation of intestinal bacteria and in the induction of a metabolic inflammation wich promotes insulin resistance, abdominal obesity development and type 2 diabetes. The aim of this study is to explore this hypothesis in human to have therapeutic solution later.
Study Type
OBSERVATIONAL
Enrollment
121
Ileon and colon biopsy
CHU de Toulouse
Toulouse, France
Number of lymphocyte TCD4 Th17 in colon sample
After colon biopsy, lymphocytes TCD4 Th17 are counted in biopsy sample.
Time frame: Day 1
Number of lymphocyte TCD4 Th17 in ileon sample
After ileon biopsy, lymphocytes TCD4 Th17 are counted in biopsy sample.
Time frame: Day 1
Number of CD45+ cells in colon sample
After colon biopsy, CD45+ cells are counted in biopsy sample.
Time frame: Day 1
Number of CD45+ cells in ileon sample
After ileon biopsy, CD45+ cells are counted in biopsy sample.
Time frame: Day 1
Activation of CD45+ cells in colon sample
After colon biopsy, CD45+ cells are analysed to see their state of activation in biopsy sample.
Time frame: Day 1
Activation of CD45+ cells in ileon sample
After ileon biopsy, CD45+ cells are analysed to see their state of activation in biopsy sample.
Time frame: Day 1
Microbiota sequencing in blood sample
Microbiota will be sequencing in blood sample
Time frame: Day 1
Microbiota quantification in blood sample
Microbiota will be quantified in blood sample
Time frame: Day 1
Microbiota sequencing in ileon sample
Microbiota will be sequencing in ileon sample
Time frame: Day 1
Microbiota sequencing in colon sample
Microbiota will be sequencing in colon sample
Time frame: Day 1
Microbiota quantification in ileon sample
Microbiota will be quantified in ileon sample
Time frame: Day 1
Microbiota quantification in colon sample
Microbiota will be quantified in colon sample
Time frame: Day 1
Bacteria production
Culture of biological samples to study the bacteria production
Time frame: Day 1
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