Staphylokinase and ABO Group Phenotype: New Players in Staphylococcus Aureus Implant-associated Infections Development

Nantes University Hospital88 enrolled

Overview

The purpose of this study is to identify bacterial and/or clinical features involved in the pathogenesis of Staphylococcus aureus implant-associated infections (IAI). Materials \& methods: In total, 57 IAI S. aureus and 31 nasal carriage (NC) S. aureus isolates were studied. Staphylococcus aureus genetic background was obtained by microarray analysis. Multilocus sequence typing was performed to determine clonal complexes (CC). Biofilm production was investigated by resazurin and crystal violet methods

Study Type

OBSERVATIONAL

Enrollment

Conditions

Staphylococcal Infection

Interventions

IAI infectionOTHER

Staphylococcus aureus nasal carriage infectionOTHER

Eligibility

Sex: ALL

Medical Language ↔ Plain English

Inclusion Criteria: * infected patients with S. Aureus IAI infection * noninfected patients screened for S. aureus nasal colonization at the orthopedic consultation ward Exclusion Criteria: * minor

Outcomes

Primary Outcomes

biofilm production measurement in the two groups

The primary aim of this study is to identify phenotypic (biofilm production) markers in two different groups of S. aureus strains isolated from patients with IAI or screened from nasal carrier in noninfected patients undergoing orthopedic surgery. By using resazurin method, IAI and NC nasal carriage isolates were divided into three groups. Isolates were classified as strong, moderate and weak biofilm producers.

Time frame: september 2012

Clonal complexes distribution

The primary aim of this study is to identify genotypic markers in two different groups of S. aureus strains isolated from patients with IAI or screened from nasal carrier in noninfected patients undergoing orthopedic surgery. IAI and NC isolates genetic background was determined in order to compare the prevalence of individual virulence factor genes. All genes provided by the Alere StaphyType DNA microarray were studied.

Time frame: september 2012

IAI and NC nasal carriage isolates genetic background

IAI and NC isolates genetic background was determined in order to compare the prevalence of individual virulence factor genes. All genes provided by the Alere StaphyType DNA microarray were studied

Time frame: september 2012

Secondary Outcomes

biofilm production correlation with genotype

quantile-quantile (QQ)-plot analysis

Time frame: december 2013

Correlation between Clonal Complexes distribution , genotype and clinical parameters

In order to determine whether clusters of S. aureus could be associated with clinical characteristics of the patients, search of association between CCs and clinical data was performed

Time frame: december 2013

Data from ClinicalTrials.gov

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