Exploratory Study of Cellular Reservoirs in Blood From HIV Infected Patients

Centre Hospitalier Universitaire de Besancon41 enrolled

Overview

This study evaluates the impact of highly active antiretroviral therapy on the size of the latent viral reservoirs in resting CD4+ T cells and monocytes in HIV positive patients. The activation state of the cells will be assessed, by measuring the activation of Akt, to determine its influence on the size of the viral reservoirs.

Study Type

INTERVENTIONAL

Allocation

Purpose

BASIC_SCIENCE

Masking

NONE

Enrollment

Conditions

HIV Infection

Interventions

additional blood sampleOTHER

Eligibility

Sex: ALLMin age: 18 YearsMax age: 70 Years

Medical Language ↔ Plain English

Inclusion Criteria: * HIV infected patients under Highly Active Anti-Retroviral Therapy (HAART) or Naïve from treatment Exclusion Criteria: * Age limits * Pregnancy * Legal restrictions to consent

Locations (1)

Centre Hospitalier Régional Universitaire

Besançon, France

Outcomes

Primary Outcomes

Measure of the integrated viral reservoir size

Measure by Alu-PCR. Results are given in number of integrated proviral DNA copies per 1 million cells.

Time frame: day 1

Measure of the proviral reservoir size

Measure by Total Proviral HIV DNA detection kit. Results are given in number of total proviral DNA copies per 1 million cells.

Time frame: day 1

Secondary Outcomes

Measure of Akt activation

Measure of Akt activation in live cells by flow cytometry

Time frame: day 1

Data from ClinicalTrials.gov

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