The purpose of this project is to study the presence of expression differences - at RNA and protein level - of different members of the tachykinin family and kisspeptin and their receptors, between fertile women and infertile patients with different etiologies.
Study Type
INTERVENTIONAL
Allocation
NON_RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
NONE
Enrollment
236
Donors and patients are subjected to ovarian puncture as part of their donation procedure or IVF treatment. Oocyte are retrieved in this procedure along with granulosa and cumulus cells. Donors and patients sign an informed consent in order to donate their granulosa and cumulus cells for the study.
Instituto de Investigaciones Químicas
Seville, Spain
IVI Sevilla
Seville, Spain
Expression level of Substance P (SP)
Expression analysis at mRNA (mRNA = messenger ribonucleic acid) and protein level using techniques of real time quantitative PCR (PCR = polymerase chain reaction), immunocytochemistry and western blot
Time frame: 4 years
Expression level of neurokinin A (NKA)
Expression analysis at mRNA and protein level using techniques of real time quantitative PCR, immunocytochemistry and western blot
Time frame: 4 years
Expression level of neurokinin B (NKB)
Expression analysis at mRNA and protein level using techniques of real time quantitative PCR, immunocytochemistry and western blot
Time frame: 4 years
Expression level of hemokinin 1 (HK-1)
Expression analysis at mRNA and protein level using techniques of real time quantitative PCR, immunocytochemistry and western blot
Time frame: 4 years
Expression level of neurokinin 1 receptor (NK1R)
Expression analysis at mRNA and protein level using techniques of real time quantitative PCR, immunocytochemistry and western blot
Time frame: 4 years
Expression of neurokinin 2 receptor (NK2R)
Expression analysis at mRNA and protein level using techniques of real time quantitative PCR, immunocytochemistry and western blot
Time frame: 4 years
Expression level of neurokinin 3 receptor (NK3R)
Expression analysis at mRNA and protein level using techniques of real time quantitative PCR, immunocytochemistry and western blot
Time frame: 4 years
This platform is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional.
Expression level of kisspeptin 1 (KISS1)
Expression analysis at mRNA and protein level using techniques of real time quantitative PCR, immunocytochemistry and western blot
Time frame: 4 years
Expression level of kisspeptin 1 receptor (KISS1R)
Expression analysis at mRNA and protein level using techniques of real time quantitative PCR, immunocytochemistry and western blot
Time frame: 4 years
Patient or donor age
Years
Time frame: 4 years
Patient or donor body mass index
kg/m2
Time frame: 4 years
Years of infertility
Number of years that the patient has been trying to have a child without success
Time frame: 4 years
Estradiol level (day of ovulation induction)
Estradiol level in blood (pg/mL), measured the day in which ovulation is induced
Time frame: 4 years
Progesterone level (day of ovulation induction)
Progesterone level in blood (ng/mL), measured the day in which ovulation is induced
Time frame: 4 years
Number of oocytes retrieved in the ovarian puncture
Time frame: 4 years
Number of mature oocytes (metaphase II) obtained in the ovarian puncture
Time frame: 4 years
Fertilization rate
Proportion of oocytes correctly fertilized (2 pronuclei and 2 polar bodies) after the ICSI (ICSI = intracytoplasmic sperm injection) procedure
Time frame: 4 years
Embryo quality
Embryo quality at day 3 of embryo development (of each embryo of the cohort). Quality assessed according to ASEBIR (ASEBIR = "Asociación Española para el estudio de la Biología de la Reproducción" = Spanish society for reproductive biology) criteria. Embryo quality grade A to D.
Time frame: 4 years
Daily and total dose of FSH (FSH = follicle stimulating hormone)
Daily dose of FSH for controlled ovarian stimulation. International Units (IU)
Time frame: 4 years
Daily and total dose of HMG (HMG = human menopausal gonadotropin)
Daily dose of HMG for controlled ovarian stimulation. International Units (IU)
Time frame: 4 years
Type of ovulation induction
Ovulation induction using 6500 IU of hCG (hCG = human chorionic gonadotropin) or 0.2 mg of triptorelin (Decapeptyl)
Time frame: 4 years