To investigate the secretion of gut hormones, in particular glucagon-like peptide 1 (GLP-1), after ingestion of glucose and fructose as separate monosaccharides or combined in the disaccharides sucrose and isomaltulose and the effect of acarbose in gastric bypass operated subjects and BMI-matched controls.
Disaccharides such as sucrose or isomaltulose (also called palatinose) require digestion into monosaccharides (both are digested to glucose and fructose) by enzymes located in the brush border of the small intestine to be absorbed. The study will investigate the influence of carbohydrate digestion on gut hormone secretion.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
SINGLE
Enrollment
20
Ingestion of the separate monosaccharides glucose (25 g) and fructose (25 g) dissolved in 200 mL of water. The load provides 0.1385 mol glucose and 0.1385 mol fructose.
Ingestion of the disaccharide sucrose (47.5 g) dissolved in 200 mL of water. The load provides 0.1385 mol glucose and 0.1385 mol fructose.
Ingestion of the slowly digestable disaccharide isomaltulose (47.5 g) dissolved in 200 mL of water. The load provides 0.1385 mol glucose and 0.1385 mol fructose.
Dept. of Endocrinology
Hvidovre, Denmark
Within group difference in GLP-1 secretion (evaluated by iAUC).
Comparison of the GLP-1 responses (evaluated by iAUC) induced by the different carbohydrate loads.
Time frame: 0-240 min following carbohydrate ingestion
Between group comparison of GLP-1 secretion (evaluated by iAUC).
GLP-1 secretion (evaluated by iAUC) in gastric bypass operated patients compared to BMI-matched control subjects for each of the carbohydrate loads.
Time frame: 0-240 min following carbohydrate ingestion
Between group comparison of differences in GLP-1 secretion (evaluated by iAUC).
Differences in GLP-1 secretion (evaluated by iAUC) induced by the different carbohydrate loads in gastric bypass operated patients compared to differences in BMI-matched control subjects.
Time frame: 0-240 min following carbohydrate ingestion
Responses (evaluated by iAUC) of glucose within and between groups.
Time frame: 0-240 min following carbohydrate ingestion
Responses (evaluated by iAUC) of insulin within and between groups.
Time frame: 0-240 min following carbohydrate ingestion
Responses (evaluated by iAUC) of C-Peptide within and between groups.
Time frame: 0-240 min following carbohydrate ingestion
Responses (evaluated by iAUC) of peptide YY (PYY) within and between groups.
Time frame: 0-240 min following carbohydrate ingestion
Responses (evaluated by iAUC) of Gastric Inhibitory Peptide (GIP) within and between groups.
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Ingestion of the disaccharide sucrose (47.5 g) and the alpha glucosidase inhibitor acarbose dissolved in 200 mL of water. The load provides 0.1385 mol glucose and 0.1385 mol fructose.
Time frame: 0-240 min following carbohydrate ingestion
Responses (evaluated by iAUC) of glucagon within and between groups.
Time frame: 0-240 min following carbohydrate ingestion
Responses (evaluated by iAUC) of oxyntomodulin within and between groups.
Time frame: 0-240 min following carbohydrate ingestion
Responses (evaluated by iAUC) of cholecystokinin (CCK) within and between groups.
Time frame: 0-240 min following carbohydrate ingestion
Responses (evaluated by iAUC) of bile acids within and between groups.
Time frame: 0-240 min following carbohydrate ingestion