Biological Markers Evaluation of Accelerated Tooth Movement

University of Sao Paulo25 enrolled

Overview

Gingival Crevicular Fluid samples will be collected using periopapers after accelerated tooth movement technique, results will be assessed with biomarkers levels done using ELISA Assays

For this prospective study, 22 individuals were selected, with skeletal discrepancies randomly divided into two groups: 1 - accelerated tooth movement technique (ATMT) and 2 - Control. Orthodontic records done in two times, initial and preoperative; Gingival Crevicular Fluid samples will be collected using periopapers in 7 times (1, 2, 7, 14, 28 days and after space closure- end of orthodontic treatment movement activation). Flapless cortical perforations will be made in ATMT Group after dental extraction. Surgical protocol will be flapless to preserve periodontal health. After extraction space closure, results will be assessed by clinical effects with biological markers levels: CCL3/MIP-1 alpha, CCL5/RANTES, CXCL8/IL8, IL-1 alpha, IL-1 beta, IL6, TNF-alpha, TRANCE/RANK L, OCN OPG IL-10 VEGF TGF-ℬ Those biomarkers levels will be performed using ELISA Assays. After collecting data, the results will be statistically analyzed using Shapiro-Wilk and, posteriorly, T-student or Wilcoxon.

Study Type

INTERVENTIONAL

Allocation

RANDOMIZED

Purpose

DIAGNOSTIC

Masking

NONE

Enrollment

Conditions

Cytokine Measurements Orthodontic Space Closure Tooth Movement

Interventions

micro-osteoperforationPROCEDURE

Eligibility

Sex: ALLMin age: 18 YearsMax age: 40 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Adult subjects ages 18-40 years old, in good general health, with adult complete dentition, regardless of presence of third molars. * Indication for orthognathic surgery * Tooth extraction indication for orthodontic treatment * Do not smoke * Periodontal and teeth healthy * Do not use bisphosphonates or corticosteroids Exclusion Criteria: * Surgically assisted rapid palatal expansion indication * Cleft lip and palate * Craniofacial syndrome * Mental disorder * Metabolic diseases

Outcomes

Primary Outcomes

Inflammatory Cytokines ad Chemokines Detection Assay

Measure the different levels of listed cytokines and chemokines after starting the anterior retraction

Time frame: 8 months

Bone remodeling markers in GCF samples

Measure the different levels of resorption biomarkers and bone formation after starting the anterior retraction

Time frame: 8 monnths

Data from ClinicalTrials.gov

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