A Biobank for Diaphragm Muscular Fiber

University Hospital, Montpellier12 enrolled

Overview

This non-interventional study focuses on mechanical ventilation used in intensive care unit to supplement ventilatory function in patients. Mechanical ventilation can "paradoxically" be at the origin of complications that can be life-threatening in patients. This muscular pathology is called ventilation-induced diaphragmatic dysfunction (DDIV). Diaphragmatic muscle collected during a digestive surgery for a benign or malignant tumor of the liver requiring surgical excision in contact with the diaphragm from the care will be conserved. The diaphragm biopsy from the care will be retained for biobanking to obtain myoblast in culture which will differentiate in Diaphragm fiber. Then these fibers will be submitted under mechanical stress condition similar to those imposed in vivo by mechanical ventilation to validate in human a model in vitro of diaphragm dysfunction induced by mechanical ventilation. Then the second part of the study will be to evaluate with this model, the efficiency of an antioxidant therapy.

Study Type

OBSERVATIONAL

Enrollment

Conditions

Diaphragm Dysfunction Mechanical Ventilation

Eligibility

Sex: ALLMin age: 30 YearsMax age: 65 Years

Medical Language ↔ Plain English

Inclusion Criteria: * age between 30 and 65 years old * patient treated by the digestive surgery department of Montpellier University Hospital for a benign or malignant tumor of the liver requiring surgical excision in contact with the diaphragm * Non-smoker patient for more than 6 months * patient clinically stable at the time of the study, ie not requiring any treatment Exclusion Criteria: * Patients undergoing treatment with antibiotics or corticosteroids, or recently within the last 4 months * Patients with a body mass index \> 30 * Patients with any criteria that may in themselves impair respiratory muscle function such as chronic obstructive pulmonary disease, heart failure, systemic infection, neuromuscular pathology, psychiatric pathology or metabolic disorder. Patients with coagulopathy or thrombocytopenia.

Outcomes

Primary Outcomes

Myoblast differentiation capacity

Description: evaluation of ROS (reactive oxygen species) production by confocal microscopie of diaphragm fiber submitted in vitro by mechanical stress.

Time frame: 24 month

Secondary Outcomes

Myoblast proliferation capacity

Description: Evaluation of diaphragm myoblast differenciation capacity in vitro under different concentration of antioxydant.