Modulation of Lactase Expression by a New PPARgamma Ligand in Duodenal Biopsies

N/ACompletedNCT02902016

University Hospital, Lille22 enrolled

Overview

This study is designed to assess the effect of a new PPARgamma modulator on the expression and activity of the lactase enzyme in human intestinal epithelial cells. Based on their previous experimental results, the investigators hypothesized that modulating intestinal PPARgamma activity is a new pharmacological mechanism allowing the control of lactase expression and activity in the gut

Study Type

INTERVENTIONAL

Allocation

Purpose

BASIC_SCIENCE

Masking

NONE

Enrollment

Conditions

Lactose Intolerance

Interventions

GEDDRUG

Four duodenal biopsies will be collected. Two biopsies will stimulated with GED (PPARgamma modulator) ex vivo during 6 hours. Two biopsies will be unstimulated (control).

Eligibility

Sex: ALLMin age: 18 YearsMax age: 70 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Subjects undergoing gastroduodenal endoscopy for digestive cancer screening * Subjects undergoing gastroduodenal endoscopy for ulcerative gastroduodenal diseases * Subjects undergoing gastroduodenal endoscopy for epigastric pains * Subjects undergoing gastroduodenal endoscopy for gastroesophageal reflux diseases Exclusion Criteria: * Subjects with macroscopic duodenal lesions detected at endoscopy * Subjects suffering from coeliac disease * Subjects suffering from atrophic gastritis * Subjects who use anticoagulant * Pregnancy or breast feeding

Locations (1)

CHRU, Hôpital Claude Huriez

Lille, France

Outcomes

Primary Outcomes

LCT mRNA expression measured by quantitative RT-PCR in duodenal biopsies stimulated ex-vivo with a new PPARgamma agonist (GED, 1mM)

Time frame: 2 years

Secondary Outcomes

LCT mRNA expression measured by quantitative RT-PCR in primary intestinal epithelial cells (isolated from duodenal biopsies) stimulated with a new PPARgamma agonist (GED, 1mM)

Time frame: 2 years

Data from ClinicalTrials.gov

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