The gut immune barrier is not fully restored in HIV-1-infected subjects despite they were receiving antiretroviral treatment. This leaky gut leads to microbial translocation from the gut lumen into the bloodstream that fuels deleterious systemic inflammation. The chemotaxis axes that allow T lymphocytes to migrate from the blood to the gut mucosa in order to reconstitute the mucosal immune barrier seems altered in treated HIV-1-infected subjects.This study aims at better understanding the mechanisms involved in this lack of mucosal immune restoration.
Pathophysiological study in human subjects, comparative, national, multicentric and prospective. Peripheral blood and intestinal biopsies will be collected.
Study Type
INTERVENTIONAL
Allocation
NON_RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
NONE
Enrollment
80
Blood draw and intestinal biopsies
Hôpital Purpan - Service de Médecine Interne
Toulouse, France
Hôpital Purpan - Service des maladies Infectieuses
Toulouse, France
Immune status: Measure of the frequencies of Th1 in peripheral blood and gut mucosa.
The frequencies of Th1 will be measured by flow cytometry.
Time frame: Baseline
Immune status: Measure of the frequencies of Th17 in peripheral blood and gut mucosa.
The frequencies of Th17 will be measured by flow cytometry.
Time frame: Baseline
Immune status: Measure of the frequencies of Th22 in peripheral blood and gut mucosa.
The frequencies of Th22 will be measured by flow cytometry.
Time frame: Baseline
Immune status: Quantification of cytokines in blood and gut mucosa.
The quantification of cytokines will be measured by luminex.
Time frame: Baseline
Immune status: Quantification of cytokines in blood and gut mucosa.
The quantification of cytokines will be measured by immuno-histochemistry.
Time frame: Baseline
Immune status: Quantification of chemiokines in blood and gut mucosa.
The quantification of chemiokines will be measured by luminex.
Time frame: Baseline
Immune status: Quantification of chemiokines in blood and gut mucosa.
The quantification of chemiokines will be measured by immuno-histochemistry.
Time frame: Baseline
Microbial translocation : Quantification of soluble CD14 in plasma.
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The quantification of CD 14 will be realised by Enzyme-Linked Immunosorbent Assay (ELISA).
Time frame: Baseline
Microbial translocation : Quantification of soluble soluble CD163 in plasma.
The quantification of CD163 will be realised by Enzyme-Linked Immunosorbent Assay (ELISA) .
Time frame: Baseline
Microbial translocation : Quantification of Lipopolysaccharide Binding Protein (LBP).
The quantification of Lipopolysaccharide Binding Protein will be realised by Enzyme-Linked Immunosorbent Assay (ELISA).
Time frame: Baseline
Microbial translocation : Quantification of Intestinal-type Fatty Acid-Binding Protein (I-FABP) in plasma.
The quantification of Intestinal-type Fatty Acid-Binding Protein (I-FABP) will be realised by Enzyme-Linked Immunosorbent Assay (ELISA).
Time frame: Baseline
Microbial translocation : Quantification of 16S RNA.
The quantification of 16S RNA will be realised by real-time Polymerase Chaine Reaction (qPCR).
Time frame: Baseline