Nebulized and Intravenous Colistin in Ventilator Associated-pneumonia

Phase 3TerminatedNCT02906722

Assistance Publique - Hôpitaux de Paris7 enrolled

Overview

Few antimicrobials are available to treat ventilated associated pneumonia (VAP) caused by Gram negative multi-resistant (MDR) bacteria. Colimycin often remains the only active antibiotic. The aim of the study is to demonstrate the superiority of nebulized colimycin over intravenous colimycin to treat VAP caused by Gramnegative MDR bacteria.

VAP is the most frequent nosocomial infection in critically ill patients and affects length of stay and cost in Intensive Care Unit. The increased incidence of nosocomial infections caused by MDR bacteria becomes a major health problem worldwide. Nowadays, few antimicrobials are available to treat Gram negative MDR VAP. Colimycin often remains the only active antibiotic. Treating VAP by intravenous (IV) colimycin has two main limitations: risk of renal toxicity and low tissue penetration. Nebulization of colimycin offers the possibility of generating high lung tissue concentrations, rapid bactericidal effects and low systemic accumulation in experimental models. To date however, there is no study comparing clinical effectiveness of nebulized and intravenous colimycin. We make the hypothesis that nebulized colimycin increases the clinical cure rate of VAP caused by Gram negative MDR bacteria compared to IV colimycin. Primary Objective: To demonstrate the superiority of nebulized colimycin over intravenous colimycin for treating VAP caused by Gram-negative MDR bacteria. Secondary Objectives: 1. To compare the microbiological cure rate at end of treatment 2. To compare the VAP recurrence rate after end of treatment 3. To compare the lung superinfection rate after end of treatment 4. To compare 28 day- and 90 day-mortality 5. To compare duration of mechanical ventilation 6. To compare length of ICU stay 7. To compare renal function during colimycin administration 8. To compare side effects resulting from colimycin nebulization and intravenous administration Ancillary study: In some centers, blood samples will be performed to measure colistin peak and trough plasma concentrations Study design: This is a randomized, multicenter, double-blind and phase III study 1. Randomization: Patients are randomly assigned to experimental group or control group: Control group: patients receive simultaneously intravenous colimycin and nebulized placebo. Experimental group: patients receive simultaneously nebulized colimycin and intravenous infusion of placebo. Dosing adjustment is according to renal function for intravenous infusion of colimycin or placebo. 2. Aerosol generation: Nebulization is performed with a vibrating plate nebulizer (Aeroneb® Solo) with following ventilator settings: * Constant flow and volume-controlled mode * Inspiratory/expiratory ratio of 1 * Tidal volume of 6-8 ml/kg * Respiratory frequency of 12-18/min * End-inspiratory pause of 20% To standardize nebulization procedure, a checklist form is completed by the nurse in charge of the patient. 3. Duration of treatment: * 10 days in each group * In intubated patients, weaning test is authorized after 4 days of treatment. If patient is extubated, aerosols are discontinued whereas intravenous infusions are continued (placebo or antibiotic) until day 10 * In tracheostomized patients: 10-day treatment for nebulized and intravenous therapy 4. Combined intravenous administration of other antimicrobials are authorized 5. Serum and microbiological samples * Serum creatinine measured daily from baseline to day 11 * Lower respiratory tract specimens at day 5 and day 11 in intubated patients 6. Survival follow-up at day 28 and 90 days Study population: Adult mechanical ventilated patients with VAP caused by Gram-negative MDR bacteria. Sample size and Power consideration: Data will be analyzed with triangular test. Assuming a clinical cure rate at day 11 of 65% in the group treated with nebulized colimycin and of 45% in the group treated with intravenous colimycin, a mean sample size of 134 patients is required to provide 80% power, with a two-sided type I error rate of 5%. The 90th percentile of the number of patients to include is 196.

Study Type

INTERVENTIONAL

Allocation

RANDOMIZED

Purpose

TREATMENT

Masking

DOUBLE

Enrollment

Conditions

Ventilator-associated Pneumonia

Interventions

Intravenous colimycinDRUG

administration once, twice or 3 times per day according to renal function

Intravenous placeboDRUG

administration once, twice or 3 times per day according to renal function

Nebulized colimycinDRUG

Nebulization is performed with a vibrating plate nebulizer (Aeroneb® Solo) every 8h

Eligibility

Sex: ALLMin age: 18 Years

Medical Language ↔ Plain English

Inclusion criteria * Age older than 18 yr * Invasive mechanical ventilation for more than 48 h Tracheostomized patients receiving intermittent mechanical ventilation can be included * VAP caused by Gram-negative MDR bacteria resistant to all β-lactams and fluoroquinolones Exclusion criteria * Extrapulmonary Gram-negative MDR infection requiring intravenous colimycin * VAP associated with bacteremia requiring combined treatment by nebulized and intravenous colimycin * Hypersensitivity to colistimethate, colistin base, polymyxins and/or their excipients * Porphyria * Severe hypoxemia defined as PaO2 / FiO2\< 100; if veno-venous ECMO is initiated, the patient can be included * Severe brain injury (initial Glasgow coma score \< 8) during the first 7 days before randomization * Myasthenia * cystic fibrosis * Refusal to participate in the study * Participation in any clinical study of a therapeutic investigational product within 30 days prior to the first day of inclusion * No affiliation to social health insurance * Patient under guardianship * Pregnancy

Locations (1)

Hôpital Pitié-Salpêtriere

Paris, France

Outcomes

Primary Outcomes

Clinical cure of VAP caused by Gram-negative multidrug resistant bacteria

Clinical cure is defined as: * resolution of clinical and biological signs of infection and improved radiological signs * and modified clinical pulmonary infection score (CPIS) less than 6 * and negative culture of lower respiratory tract specimen or, successful weaning from invasive mechanical ventilation between day 5 and day 11 * patient colonized with the same pathogen in the respiratory tract is considered as cured, if clinical and biological signs of infection resolved, radiological signs improved, CPIS \< 6 and/or successful weaning from invasive mechanical ventilation has been obtained.

Time frame: At end of therapy visit (day11) or before day11 if treatment is considered as failed.

Secondary Outcomes

Microbiological cure rate

Time frame: At end of therapy visit (day11) or before day11 if treatment is considered as failed.

VAP recurrence rate

VAP recurrence rate, defined as initial clinical cure of VAP with colimycin at day 11 followed by reappearance of clinical and biological signs of infection, CPIS greater than 6, and significant concentrations of Gram-negative MDR bacteria in lower respiratory tract specimen

Time frame: day 28

Lung superinfection rate

Lung superinfection rate defined as reappearance of VAP caused by pathogens other than Gram-negative MDR bacteria isolated from lower respi¬ratory tract specimens from day 11 to day 28

Time frame: day 11, day 28

Mortality

Time frame: day 28 and day 90

Duration of mechanical ventilation

Time frame: Participants will be followed for the duration of ICU stay, an expected average of 3 weeks

Data from ClinicalTrials.gov

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