Cases which undergone ICSI before with failure of fertilization will do another ICSI cycle with removal of the polar body just before the ICSI procedure and sperm injection at the site of polar body removal. This study group will be evaluated with another group with the classic ICSI procedure
Patients with previous fertilization failure will be divided into 2 groups: Group 1 (control): Will undergo classic ICSI procedure in half the MII oocyte of the same case. Group 2 (study group): The other half of the MII oocyte of the same case will undergo: 1. Polar body removal. 2. ICSI procedure with injection of the spermatozoon in the same zona opening (done by laser for polar body removal). Results: Will be collected with evaluation of number of 2PN zygotes, Day 1, Day 2, Day 3, Day 5 embryos in all the cases.
Study Type
INTERVENTIONAL
Allocation
NA
Purpose
TREATMENT
Masking
NONE
Enrollment
40
zona drilling with laser followed by polar body removal then ICSI procedure
Classic ICSI case was done be injecting sperm inside Oocyte while polar body is already existed at 6 or 12 O'clock position.
Banha University- Hawaa Fertility center
Banhā, Qalyubia Governorate, Egypt
fertilization rate of the zygotes
fertilization check is done under inverted microscopy by 2PN check
Time frame: 16-20 hours post ICSI
division rate of the embryos by the number of the blastomeres.
Detection of the number of the blastomeres and quality of the embryos under inverted microscopy. At day 5, detection of the rate of the blastocyst formation and quality of the embryos.
Time frame: Day 2 & 3 & 5
This platform is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional.