This study test whether a Continuous Glucose Monitor can pickup differences in glucose (in the interstitial fluid) during a dietary intervention using meals with either a high with a low glycemic load.
The aim of this study is to determine the suitability of a continuous glucose monitor to detect the impact of a dietary intervention on postprandial blood glucose levels in normal, healthy, free living subjects. We will compare a dietary intervention of meals with a high glycemic load (high post-meal blood glucose) versus meals with a low glycemic load (low post-meal blood glucose) in a cross-over study. In addition, the glucose profiles obtained both via venous blood sampling and via continuous glucose monitor following a standard carbohydrate load will be compared.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
SINGLE
Enrollment
23
Low glycemic load diets will be consumed at breakfast, lunch and dinner for three consecutive days
High glycemic load diets will be consumed at breakfast, lunch and dinner for three consecutive days
Maastricht University
Maastricht, Netherlands
Post prandial glucose concentration
Difference in glucose concentrations between the low glycemic load and the high glycemic load diet. Incremental area under the curve after the three main meals (breakfast, lunch, dinner) during both dietary interventions will be calculated. The nine post prandial periods for both low and high glycemic load diet per subject will be combined in the mixed model.
Time frame: 0 - 120 minutes after the start of each of the 3 main meals (breakfast, lunch dinner)
Three-day glucose concentration
Difference in glucose concentrations between the low and the high glycemic load diet. Total area under the curve will be compared
Time frame: From start of dietary intervention until the end 3 days later
Day time glucose concentration
Difference in glucose concentrations between the low and the high glycemic load diet. Total area under the curve will be compared
Time frame: Between 07:00-22:00 h
Night time glucose concentration
Difference in glucose concentrations between the low and the high glycemic load diet. Total area under the curve will be compared
Time frame: Between 22:01-26:59 h
Glucose variability
The Continuous Overall Net Glycemic Action (CONGA) will be calculated.
Time frame: From start of dietary intervention until the end 3 days later
Comparison of continuous interstitial glucose with venous blood glucose
Bland-Altman plots of venous blood glucose plotted against the continuous interstitial glucose at the closest available time point. Standard meal will be 200 grams of rice consumed on the day after both the low and high glycemic load diets
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Time frame: -30, -15, 15, 30, 45, 60, 90 and 120 minutes after consumption of a standard meal