The aim of this study is to assess the clinical effect of aPDT on deep caries in permanent teeth. Half of the deep caries will receive aPDT disinfect in restorative treatment procedure, while the other half will receive sodium 0.9% saline in restorative treatment procedure.
The aim of the proposed study is to assess the clinical effect of antimicrobial photodynamic therapy (aPDT) using LED on dentin with deep caries in permanent teeth. Permanent teeth with deep carious lesions on the occlusal surface indicated for restorative treatment will be randomly allocated to two groups: Group 1 -Control after incomplete caries removal, washed with sodium 0.9% saline; Group 2 - PDT + CH after incomplete caries removal, disinfect the residual dentin with PDT(LED + O-Toluidine Blue). Dentin samples will be collected before and after disinfect for microbiological analysis. Then the teeth will be capped with CH and restored using resin with clinical and radiographic follow up at 6, 12 and 24 months.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
TREATMENT
Masking
DOUBLE
Enrollment
40
Local anesthesia was performed. Tooth was isolated using a rubber dam isolation in order to collect the dentin samples.
Partial carious tissue was removed with conventional dentinal curette and collection of dentin with a spoon (1#) was performed.
The remaining dentin was dipped in sodium 0.9% saline for 2min.
School and Hospital of Stomatology, Fujian Medical University
Fuzhou, Fujian, China
RECRUITINGQuantification of bacteria by colony counting
Quantification of total bacteria
Time frame: 2 days after dentin collection
Tooth Pain
Patients reported the presence or absence of tooth pain
Time frame: 1 day and 6, 12, 24 months
Restoration Quality
The quality of restoration was evaluated through the absence of radiographic radiolucent area
Time frame: 1 day and 6, 12, 24 months
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The remaining dentin was disinfected with aPDT for 2 min.
A new dentin collection was performed after this treatment at another site of the cavity. Then teeth were conventionally restored using the following materials: calcium hydroxide lining material and resin (Z350,3M).