The project aims to study the effects of inclusion of sunflower oil, vitamin E and selenium in the diet of dairy cows on milk composition and the influence of modified milk on nutrition and health of elderly. To achieve those goals, two experiments will be conducted, both lasting 12 weeks. In the first one, 32 cows at the beginning of lactation will be allotted in a completely randomized block design with a 2 x 2 factorial arrangement of treatments, as follow: 1) control diet; 2) control diet plus selenium and vitamin E; 3) control diet plus sunflower oil diet; 4) control diet plus sunflower oil, selenium and vitamin E. This experiment seeks the development of modified milks through nutritional manipulation of cow's diet. Concomitantly, in the second experiment, 132 elderly people will receive the four different kinds of milk produced in the first experiment in a completely randomized block (gender) design. The selection of the elderly for each group shall be composed clinical evaluations. Will be conducted biochemical tests before and after supplementation of milk consumption (serum α-tocopherol, selenium, analysis of fatty acid profile). Assessment of nutritional status will be made body mass index (BMI) with each participant before and after the experiment of 12 weeks, including body composition by multifrequency bioelectrical impedance analysis (BIA), evaluating the strength of pressure of the hand portable dynamometer, demographics and food consumption. The study of human nutrition and health will be held in the form of a double-blind study. The second experiment aims to study nutrition and health of elderly people, as it is expected that milk produced with better fatty acid profile, vitamin and minerals can improve the inflammatory profile of markers of the elderly. This project is important for the human population in general, particularly elderly people, health workers, human and animal nutritionists, and especially for the milk chain, as it has a potential to develop new dairy products and boost milk consumption.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
OTHER
Masking
QUADRUPLE
Enrollment
132
There is no intervention pre-specified before the institutionalized elderly to receive three times per day of milk (total 600ml of milk all day) produced by lactating cows fed with the following diets: control (C)+selenium and vitamin E (A).
There is no intervention pre-specified before the institutionalized elderly to receive three times per day of milk (total 600ml of milk all day) produced by lactating cows fed with the following diets: control (C)+sunflower oil (O).
There is no intervention pre-specified before the institutionalized elderly to receive three times per day of milk (total 600ml of milk all day) produced by lactating cows fed with the following diets:control (C)+selenium, vitamin E and sunflower oil (AO).
There is no intervention pre-specified before the institutionalized elderly to receive three times per day of milk (total 600ml of milk all day) produced by lactating cows fed with the following diets: control (C)
Clinics Hospital of the Ribeirao Preto Medical School
Ribeirão Preto, São Paulo, Brazil
Change in serum α-tocopherol
Assessment by blood sampling. Collect of α-tocopherol before and after to 12 weeks of milk supplementation
Time frame: before and after 12 weeks
Change in serum selenium
Assessment by blood sampling. Collect of selenium before and after to 12 weeks milk supplementation
Time frame: before and after 12 weeks
Change in analysis of fatty acid profile
Assessment by blood sampling. Collect of analysis of fatty acid profile before and after to 12 weeks of milk supplementation
Time frame: before and after 12 weeks
Assessment of nutritional status: food intake questionnaire
Food consumption questionnaire during the milk supplementation
Time frame: before and after 12 weeks
Change in handgrip strength
Measure of the hand grip strength using a portable dynamometer before and after 12 weeks of milk supplementation
Time frame: before and after 12 weeks
Assessment of nutritional status: anthropometry (BMI)
BMI before and after 12 weeks of milk supplementation
Time frame: before and after 12 weeks
Assessment of nutritional status: body composition
Body composition measured by multifrequency bioelectrical impedance analysis (BIA) before and after to 12 week of milk supplementation
Time frame: before and after 12 weeks
Assessment demographics: questionnaire
Demographics questionnaire applied during the milk supplementation
Time frame: before and after 12 weeks
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