RNA and Heat Shock Protein Biomarkers in Radiation-induced Fibrosis in Breast Cancer

Inclusion Criteria: * women * age ≥ 18 and \<70 years old * non metastatic disease * ECOG performance status 0 or 1 * chest size ≤ 110 cm et bra size \<D * absence of reconstructive breast surgery * patient able to undergo blood samples (haematological conditions allowing blood sample) * non-evolving carcinological disease * absence of systemic inflammatory disease (other than scleroderma) or diabetes * no inflammatory ou infectious flare on biopsy site at the time of inclusion * invasive or in situ breast carcinoma * ability to provide an informed written consent form * affiliation to a social security system Then stratification into two groups : group 1 : radio-sensitive patients * Post-operative radiotherapy completed at least 6 months ago AND * radiation induced dermal and/or soft tissue toxicity (dermatitis, fibrosis, atrophy) rated \> 2 (CTCAE v4.0 scale) group 2 : radio-tolerant (control) patients * Post-operative radiotherapy completed more than 4 years ago AND * radiation induced dermal and/or soft tissue toxicity (dermatitis, fibrosis, atrophy) rated ≤1 (CTCAE v4.0 scale) . Exclusion Criteria: * age \<18 or \> 70 years old * evolutive cancer / metastatic disease * chest size \> 110 cm et bra size ≥ D * previous reconstructive breast surgery * ECOG performance status \> 1 * systemic inflammatory disease or diabetes * inflammatory ou infectious flare on biopsy site at the time of inclusion, very significant ulceration in the treated breast * anemic patients * use of oral anticoagulants * pregnant or likely to be in 6 months * patients deprived of liberty or under supervision * non-affiliation to a social security system