Skeletal Muscle Energy Metabolism in Women With Weight Loss and Ovarian and/or Endometrial Cancer With Weight Loss

University Hospital, Tours70 enrolled

Overview

the aim is to study skeletal muscle metabolism alterations métaboliques associated with weight loss in women with ovarian and/or endometrial cancer according to BMI.

Study Type

INTERVENTIONAL

Allocation

NON_RANDOMIZED

Purpose

HEALTH_SERVICES_RESEARCH

Masking

NONE

Enrollment

Conditions

Weight Loss Ovarian Carcinoma Endometrial Carcinoma

Interventions

SamplesOTHER

Blood samples, muscle biopsy, adipose tissue samples and ovarian cancer tumor sample

Eligibility

Sex: FEMALEMin age: 18 Years

Medical Language ↔ Plain English

Case group selection criteria: Inclusion Criteria: * Women aged 18 years old and more * Ovarian and/or endometrial cancer * weight loss related to cancer (weight loss \> 5% weight in one month or 10% in 6 months) * Indication to therapeutic surgery * Women affiliated to social security scheme * Women who can understand French and able to sign Informed consent Exclusion Criteria: * Unbalanced Diabetes * Chronic neuromuscular disorder * Any severe uncontrolled medical condition Control group selection criteria: Inclusion Criteria: * Women aged 18 years and more * Surgery Indication for a benign endometrial and/ or ovarian disease * Patient affiliated to social security scheme * Women who can understand French and able to sign Informed consent Exclusion Criteria: * Unbalanced Diabetes * Chronic neuromuscular disorder * Any severe uncontrolled medical condition

Locations (1)

Gynecology Department, University Teaching Hospital, Tours

Tours, France

Outcomes

Primary Outcomes

Measurement of muscle mitochondrial bioenergetics according to BMI

Measurement of muscle mitochondrial bioenergetics by high resolution oxygraphy

Time frame: muscle biopsy realized during surgery

Secondary Outcomes

on muscle biopsy

* Quantification of lipids on electron microscope * Type of muscular fibers * Analyze of gene expression and proteins involved in 1/ Mitochondrial metabolism 2/ lipid metabolism and 3/ muscular proteolysis by RTqPCR with SyBR Green and Western Blot

Time frame: muscle biopsy realized during surgery

on white adipose tissue

adipose tissue composition (fatty acids quantification, sterols, steroids) * RT-qPCR quantification of molecules involved in lipid metabolism (synthesis, transport) and in inflammation (immune profile and cytokines) * RTqPCR analysis, western blot and immunohistochemical expression of markers of transformation into brown adipose tissue * in specific milieu, in vitro experimentation on skeletal muscular cells

Time frame: adipose tissue samples taken during surgery

on ovarian cancer tumor samples

* lipid composition (fatty acids quantification, sterols, steroids) * RT-qPCR quantification of molecules involved in lipid metabolism (synthesis, transport) and in inflammation (immune profil and cytokines) * culture of tumour cells to evaluate resistance to chemotherapy (platinum)

Time frame: ovarian tumor biopsies realized during surgery

on computerized tomography

assessment of body composition (fat and muscle compartments)

Time frame: 1 day of abdominal CT scan imaging procedure performed at diagnosis

Data from ClinicalTrials.gov

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