Release of Nociceptin From Granulocytes in Sepsis

Inclusion Criteria: * For septic patients; 1. Participant is willing and able to give informed consent for participation in the study, or if lacking capacity, a next of kin or advocate is willing and able to give assent for participation in the study. Must be able to read and understand English. 2. Male or Female, aged 18 years or above. 3. Diagnosed with sepsis and admitted to the intensive care unit. 4. Able (in the Investigators opinion) and willing to comply with all study requirements. 5. Willing to allow his or her General Practitioner and consultant, if appropriate, to be notified of participation in the study. Healthy Volunteers; 1. Participant is willing and able to give informed consent for participation in the study. Must be able to read and understand English. 2. Male or Female, aged 18 years or above and be 3. In good health. 4. Have had no course of medication, whether prescribed or over-the-counter, in the four weeks before first study dose and no individual doses in the final two weeks other than mild analgesia, vitamins and mineral supplements or, for females, oral contraceptives 5. Able (in the Investigators opinion) and willing to comply with all study requirements. 6. Willing to allow his or her General Practitioner and consultant, if appropriate, to be notified of participation in the study. Exclusion Criteria: * 1\. Conditions which may make phlebotomy hazardous to the participant (such as significant bleeding disorders or anaemia, or allergy), or to the investigator (blood viral infection). 2\. Any significant disease or disorder which, in the opinion of the Investigator, may either put the participants at risk because of participation in the study, or may influence the result of the study, or the participant's ability to participate in the study. 3\. Participants who have participated in another research study involving an investigational product in the past 12 weeks.