Introduction: Ovarian hyperstimulation syndrome (OHSS) is an iatrogenic phenomenon, poorly understood and difficult to predict, complicating intense ovarian stimulation cycle. The most severe symptoms, which associate vascular permeability disorders and hypercoagulability, occur in 0.2 to 1% of the cases and often require intensive care. Activation of endothelial, platelet, erythrocyte or leukocyte cells trigger the release of small specific vesicles, called microparticles, used as markers. Classically leading to endothelial dysfunction and hypercoagulability, the endothelial activation phenomenon could constitute the main cause of OHSS or help predict its severity, as established for various other diseases (cerebral stroke, infarct and lupus…). However, so far, this endothelial activation role has never been studied. Objectives: Evaluate the serum level of microparticles as a predictor of adverse outcomes; correlate it to hypercoagulability and changes of endothelial permeability associated with this syndrome. Methodology: Prospective Pilote Cohort study, evaluating before and throughout the ovarian stimulation cycle (6 samples/patient), the serum modulation of: * Endothelial activation markers (endothelial-derived microparticles, E-selectin) * Procoagulant markers (microparticles from platelet, erythrocyte or leukocyte origin, Von Willbrand factor, thrombin-antithrombin complex, prothrombin fragment 1+2) * Endothelial disjunction marker (soluble CD 146) A group of 50 patients will be assessed Techniques: Flow cytometry for measurement of microparticles expressing non specific (Annexin V) and cell specific surface determinants (CD 31, CD 41, CD 45 or glycophorin A). Use of commercial kits for other serum markers.
Study Type
OBSERVATIONAL
Enrollment
50
Quantification of Microparticles subsets from endothelial origin in the circulating blood
Venipuncture for blood sampling and exam with flow cytometry
Time frame: At day 3 of an unstimulated (i.e. natural) menstrual cycle, before IVF cycle, when basal hormonal assessments is performed for fertility work-up
Quantification of Microparticles subsets from erythrocyte origin in the circulating blood
Venipuncture for blood sampling and exam with flow cytometry
Time frame: At day 3 of an unstimulated (i.e. natural) menstrual cycle, before IVF cycle, when basal hormonal assessments is performed for fertility work-up
Quantification of Microparticles subsets from leukocyte origin in the circulating blood
Venipuncture for blood sampling and exam with flow cytometry
Time frame: At day 3 of an unstimulated (i.e. natural) menstrual cycle, before IVF cycle, when basal
Quantification of Microparticles subsets from platelet origin in the circulating blood
Venipuncture for blood sampling and exam with flow cytometry
Time frame: At day 3 of an unstimulated (i.e. natural) menstrual cycle, before IVF cycle, when basal hormonal assessments is performed for fertility work-up
Quantification of Tissue Factor-Dependent Procoagulant Activity (MP-TF) in the circulating blood
Venipuncture for blood sampling and exam with home made device
Time frame: At day 3 of an unstimulated (i.e. natural) menstrual cycle, before IVF cycle, when basal
Quantification of Plasmin Generation Capacity (MP-PGC) in the circulating blood
Venipuncture for blood sampling and exam with home made device
Time frame: At day 3 of an unstimulated (i.e. natural) menstrual cycle, before IVF cycle, when basal
Quantification of Fibrin monomer in the circulating blood
Venipuncture for blood sampling and exam with commercial device
Time frame: At day 3 of an unstimulated (i.e. natural) menstrual cycle, before IVF cycle, when basal hormonal assessments is performed for fertility work-up
Quantification of D-dimer in the circulating blood
Venipuncture for blood sampling and exam with commercial device
Time frame: At day 3 of an unstimulated (i.e. natural) menstrual cycle, before IVF cycle, when basal hormonal assessments is performed for fertility work-up
Quantification of E-selectin in the circulating blood
Venipuncture for blood sampling and exam with commercial device
Time frame: At day 3 of an unstimulated (i.e. natural) menstrual cycle, before IVF cycle, when basal hormonal assessments is performed for fertility work-up
Quantification of soluble CD 146 in the circulating blood
Venipuncture for blood sampling and exam with commercial device
Time frame: At day 3 of an unstimulated (i.e. natural) menstrual cycle, before IVF cycle, when basal hormonal assessments is performed for fertility work-up
Quantification of Von Willbrand factor in the circulating blood
Venipuncture for blood sampling and exam with commercial device
Time frame: At day 3 of an unstimulated (i.e. natural) menstrual cycle, before IVF cycle, when basal hormonal assessments is performed for fertility work-up
Quantification of thrombin-antithrombin complex in the circulating blood
Venipuncture for blood sampling and exam with commercial device
Time frame: At day 3 of an unstimulated (i.e. natural) menstrual cycle, before IVF cycle, when basal hormonal assessments is performed for fertility work-up
Quantification of prothrombin fragment 1+2 in the circulating blood
Venipuncture for blood sampling and exam with commercial device
Time frame: At day 3 of an unstimulated (i.e. natural) menstrual cycle, before IVF cycle, when basal hormonal assessments is performed for fertility work-up
Quantification of Microparticles subsets from endothelial origin in the circulating blood
Venipuncture for blood sampling and exam with flow cytometry
Time frame: During ovarian stimulation for IVF (first cycle), at first day of FSH stimulation
Quantification of Microparticles subsets from erythrocyte origin in the circulating blood
Venipuncture for blood sampling and exam with flow cytometry
Time frame: During ovarian stimulation for IVF (first cycle), at first day of FSH stimulation
Quantification of Microparticles subsets from leukocyte origin in the circulating blood
Venipuncture for blood sampling and exam with flow cytometry
Time frame: During ovarian stimulation for IVF (first cycle), at first day of FSH stimulation
Quantification of Microparticles subsets from platelet origin in the circulating blood
Venipuncture for blood sampling and exam with flow cytometry
Time frame: During ovarian stimulation for IVF (first cycle), at first day of FSH stimulation
Quantification of Tissue Factor-Dependent Procoagulant Activity (MP-TF) in the circulating blood
Venipuncture for blood sampling and exam with home made device
Time frame: During ovarian stimulation for IVF (first cycle), at first day of FSH stimulation
Quantification of Plasmin Generation Capacity (MP-PGC) in the circulating blood
Venipuncture for blood sampling and exam with home made device
Time frame: During ovarian stimulation for IVF (first cycle), at first day of FSH stimulation
Quantification of Fibrin monomer in the circulating blood
Venipuncture for blood sampling and exam with commercial device
Time frame: During ovarian stimulation for IVF (first cycle), at first day of FSH stimulation
Quantification of D-dimer in the circulating blood
Venipuncture for blood sampling and exam with commercial device
Time frame: During ovarian stimulation for IVF (first cycle), at first day of FSH stimulation
Quantification of E-selectin in the circulating blood
Venipuncture for blood sampling and exam with commercial device
Time frame: During ovarian stimulation for IVF (first cycle), at first day of FSH stimulation
Quantification of soluble CD 146 in the circulating blood
Venipuncture for blood sampling and exam with commercial device
Time frame: During ovarian stimulation for IVF (first cycle), at first day of FSH stimulation
Quantification of Von Willbrand factor in the circulating blood
Venipuncture for blood sampling and exam with commercial device
Time frame: During ovarian stimulation for IVF (first cycle), at first day of FSH stimulation
Quantification of thrombin-antithrombin complex in the circulating blood
Venipuncture for blood sampling and exam with commercial device
Time frame: During ovarian stimulation for IVF (first cycle), at first day of FSH stimulation
Quantification of prothrombin fragment 1+2 in the circulating blood
Venipuncture for blood sampling and exam with commercial device
Time frame: During ovarian stimulation for IVF (first cycle), at first day of FSH stimulation
Quantification of Microparticles subsets from endothelial origin in the circulating blood
Venipuncture for blood sampling and exam with flow cytometry
Time frame: During ovarian stimulation for IVF (first cycle), at ovarian triggering day, i.e.when ≥ 3 ovarian follicles rich 17mm
Quantification of Microparticles subsets from erythrocyte origin in the circulating blood
Venipuncture for blood sampling and exam with flow cytometry
Time frame: During ovarian stimulation for IVF (first cycle), at ovarian triggering day, i.e.when ≥ 3 ovarian follicles rich 17mm
Quantification of Microparticles subsets from leukocyte origin in the circulating blood
Venipuncture for blood sampling and exam with flow cytometry
Time frame: During ovarian stimulation for IVF (first cycle), at ovarian triggering day, i.e.when ≥ 3 ovarian follicles rich 17mm
Quantification of Microparticles subsets from platelet origin in the circulating blood
Venipuncture for blood sampling and exam with flow cytometry
Time frame: During ovarian stimulation for IVF (first cycle), at ovarian triggering day, i.e.when ≥ 3 ovarian follicles rich 17mm
Quantification of Tissue Factor-Dependent Procoagulant Activity (MP-TF) in the circulating blood
Venipuncture for blood sampling and exam with home made device
Time frame: During ovarian stimulation for IVF (first cycle), at ovarian triggering day, i.e.when ≥ 3 ovarian follicles rich 17mm
Quantification of Plasmin Generation Capacity (MP-PGC) in the circulating blood
Venipuncture for blood sampling and exam with home made device
Time frame: During ovarian stimulation for IVF (first cycle), at ovarian triggering day, i.e.when ≥ 3 ovarian follicles rich 17mm
Quantification of Fibrin monomer in the circulating blood
Venipuncture for blood sampling and exam with commercial device
Time frame: During ovarian stimulation for IVF (first cycle), at ovarian triggering day, i.e.when ≥ 3 ovarian follicles rich 17mm
Quantification of D-dimer in the circulating blood
Venipuncture for blood sampling and exam with commercial device
Time frame: During ovarian stimulation for IVF (first cycle), at ovarian triggering day, i.e.when ≥ 3 ovarian follicles rich 17mm
Quantification of E-selectin in the circulating blood
Venipuncture for blood sampling and exam with commercial device
Time frame: During ovarian stimulation for IVF (first cycle), at ovarian triggering day, i.e.when ≥ 3 ovarian follicles rich 17mm
Quantification of soluble CD 146 in the circulating blood
Venipuncture for blood sampling and exam with commercial device
Time frame: During ovarian stimulation for IVF (first cycle), at ovarian triggering day, i.e.when ≥ 3 ovarian follicles rich 17mm
Quantification of Von Willbrand factor in the circulating blood
Venipuncture for blood sampling and exam with commercial device
Time frame: During ovarian stimulation for IVF (first cycle), at ovarian triggering day, i.e.when ≥ 3 ovarian follicles rich 17mm
Quantification of thrombin-antithrombin complex in the circulating blood
Venipuncture for blood sampling and exam with commercial device
Time frame: During ovarian stimulation for IVF (first cycle), at ovarian triggering day, i.e.when ≥ 3 ovarian follicles rich 17mm
Quantification of prothrombin fragment 1+2 in the circulating blood
Venipuncture for blood sampling and exam with commercial device
Time frame: During ovarian stimulation for IVF (first cycle), at ovarian triggering day, i.e.when ≥ 3 ovarian follicles rich 17mm
Quantification of Microparticles subsets from endothelial origin in the circulating blood
Venipuncture for blood sampling and exam with flow cytometry
Time frame: 4 days after ovarian triggering for IVF (first cycle), at the day of embryo transfer of the Day 2 embryos
Quantification of Microparticles subsets from erythrocyte origin in the circulating blood
Venipuncture for blood sampling and exam with flow cytometry
Time frame: 4 days after ovarian triggering for IVF (first cycle), at the day of embryo transfer of the Day 2 embryos
Quantification of Microparticles subsets from leukocyte origin in the circulating blood
Venipuncture for blood sampling and exam with flow cytometry
Time frame: 4 days after ovarian triggering for IVF (first cycle), at the day of embryo transfer of the Day 2 embryos
Quantification of Microparticles subsets from platelet origin in the circulating blood
Venipuncture for blood sampling and exam with flow cytometry
Time frame: 4 days after ovarian triggering for IVF (first cycle), at the day of embryo transfer of the Day 2 embryos
Quantification of Tissue Factor-Dependent Procoagulant Activity (MP-TF) in the circulating blood
Venipuncture for blood sampling and exam with home made device
Time frame: 4 days after ovarian triggering for IVF (first cycle), at the day of embryo transfer of the Day 2 embryos
Quantification of Plasmin Generation Capacity (MP-PGC) in the circulating blood
Venipuncture for blood sampling and exam with home made device
Time frame: 4 days after ovarian triggering for IVF (first cycle), at the day of embryo transfer of the Day 2 embryos
Quantification of Fibrin monomer in the circulating blood
Venipuncture for blood sampling and exam with commercial device
Time frame: 4 days after ovarian triggering for IVF (first cycle), at the day of embryo transfer of the Day 2 embryos
Quantification of D-dimer in the circulating blood
Venipuncture for blood sampling and exam with commercial device
Time frame: 4 days after ovarian triggering for IVF (first cycle), at the day of embryo transfer of the Day 2 embryos
Quantification of E-selectin in the circulating blood
Venipuncture for blood sampling and exam with commercial device
Time frame: 4 days after ovarian triggering for IVF (first cycle), at the day of embryo transfer of the Day 2 embryos
Quantification of soluble CD 146 in the circulating blood
Venipuncture for blood sampling and exam with commercial device
Time frame: 4 days after ovarian triggering for IVF (first cycle), at the day of embryo transfer of the Day 2 embryos
Quantification of Von Willbrand factor in the circulating blood
Venipuncture for blood sampling and exam with commercial device
Time frame: 4 days after ovarian triggering for IVF (first cycle), at the day of embryo transfer of the Day 2 embryos
Quantification of thrombin-antithrombin complex in the circulating blood
Venipuncture for blood sampling and exam with commercial device
Time frame: 4 days after ovarian triggering for IVF (first cycle), at the day of embryo transfer of the Day 2 embryos
Quantification of prothrombin fragment 1+2 in the circulating blood
Venipuncture for blood sampling and exam with commercial device
Time frame: 4 days after ovarian triggering for IVF (first cycle), at the day of embryo transfer of the Day 2 embryos
Quantification of Microparticles subsets from endothelial origin in the circulating blood
Venipuncture for blood sampling and exam with flow cytometry
Time frame: 9 days after ovarian triggering for IVF (first cycle), at mid-luteal phase
Quantification of Microparticles subsets from erythrocyte origin in the circulating blood
Venipuncture for blood sampling and exam with flow cytometry
Time frame: 9 days after ovarian triggering for IVF (first cycle), at mid-luteal phase
Quantification of Microparticles subsets from leukocyte origin in the circulating blood
Venipuncture for blood sampling and exam with flow cytometry
Time frame: 9 days after ovarian triggering for IVF (first cycle), at mid-luteal phase
Quantification of Microparticles subsets from platelet origin in the circulating blood
Venipuncture for blood sampling and exam with flow cytometry
Time frame: 9 days after ovarian triggering for IVF (first cycle), at mid-luteal phase
Quantification of Tissue Factor-Dependent Procoagulant Activity (MP-TF) in the circulating blood
Venipuncture for blood sampling and exam with home made device
Time frame: 9 days after ovarian triggering for IVF (first cycle), at mid-luteal phase
Quantification of Plasmin Generation Capacity (MP-PGC) in the circulating blood
Venipuncture for blood sampling and exam with home made device
Time frame: 9 days after ovarian triggering for IVF (first cycle), at mid-luteal phase
Quantification of Fibrin monomer in the circulating blood
Venipuncture for blood sampling and exam with commercial device
Time frame: 9 days after ovarian triggering for IVF (first cycle), at mid-luteal phase
Quantification of D-dimer in the circulating blood
Venipuncture for blood sampling and exam with commercial device
Time frame: 9 days after ovarian triggering for IVF (first cycle), at mid-luteal phase
Quantification of E-selectin in the circulating blood
Venipuncture for blood sampling and exam with commercial device
Time frame: 9 days after ovarian triggering for IVF (first cycle), at mid-luteal phase
Quantification of soluble CD 146 in the circulating blood
Venipuncture for blood sampling and exam with commercial device
Time frame: 9 days after ovarian triggering for IVF (first cycle), at mid-luteal phase
Quantification of Von Willbrand factor in the circulating blood
Venipuncture for blood sampling and exam with commercial device
Time frame: 9 days after ovarian triggering for IVF (first cycle), at mid-luteal phase
Quantification of thrombin-antithrombin complex in the circulating blood
Venipuncture for blood sampling and exam with commercial device
Time frame: 9 days after ovarian triggering for IVF (first cycle), at mid-luteal phase
Quantification of Microparticles subsets from platelet origin in the circulating blood
Venipuncture for blood sampling and exam with commercial device
Time frame: 16 days after ovarian triggering for IVF (first cycle), at the day of pregnancy test
Quantification of Microparticles subsets from endothelial origin in the circulating blood
Venipuncture for blood sampling and exam with flow cytometry
Time frame: 16 days after ovarian triggering for IVF (first cycle), at the day of pregnancy test
Quantification of Microparticles subsets from erythrocyte origin in the circulating blood
Venipuncture for blood sampling and exam with flow cytometry
Time frame: 16 days after ovarian triggering for IVF (first cycle), at the day of pregnancy test
Quantification of Microparticles subsets from leukocyte origin in the circulating blood
Venipuncture for blood sampling and exam with flow cytometry
Time frame: 16 days after ovarian triggering for IVF (first cycle), at the day of pregnancy test
Quantification of Microparticles subsets from platelet origin in the circulating blood
Venipuncture for blood sampling and exam with flow cytometry
Time frame: 16 days after ovarian triggering for IVF (first cycle), at the day of pregnancy test
Quantification of Tissue Factor-Dependent Procoagulant Activity (MP-TF) in the circulating blood
Venipuncture for blood sampling and exam with home made device
Time frame: 16 days after ovarian triggering for IVF (first cycle), at the day of pregnancy test
Quantification of Plasmin Generation Capacity (MP-PGC) in the circulating blood
Venipuncture for blood sampling and exam with home made device
Time frame: 16 days after ovarian triggering for IVF (first cycle), at the day of pregnancy test
Quantification of Fibrin monomer in the circulating blood
Venipuncture for blood sampling and exam with commercial device
Time frame: 16 days after ovarian triggering for IVF (first cycle), at the day of pregnancy test
Quantification of D-dimer in the circulating blood
Venipuncture for blood sampling and exam with commercial device
Time frame: 16 days after ovarian triggering for IVF (first cycle), at the day of pregnancy test
Quantification of E-selectin in the circulating blood
Venipuncture for blood sampling and exam with commercial device
Time frame: 16 days after ovarian triggering for IVF (first cycle), at the day of pregnancy test
Quantification of soluble CD 146 in the circulating blood
Venipuncture for blood sampling and exam with commercial device
Time frame: 16 days after ovarian triggering for IVF (first cycle), at the day of pregnancy test
Quantification of Von Willbrand factor in the circulating blood
Venipuncture for blood sampling and exam with commercial device
Time frame: 16 days after ovarian triggering for IVF (first cycle), at the day of pregnancy test
Quantification of thrombin-antithrombin complex in the circulating blood
Venipuncture for blood sampling and exam with commercial device
Time frame: 16 days after ovarian triggering for IVF (first cycle), at the day of pregnancy test
Quantification of prothrombin fragment 1+2 in the circulating blood
Venipuncture for blood sampling and exam with commercial device
Time frame: 16 days after ovarian triggering for IVF (first cycle), at the day of pregnancy test
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